谷胱甘肽S转移酶在急性早幼粒细胞白血病中的表达及临床意义  

The expression of GSTπ in acute promyelocytic leukemia and its clinical significance

作　　者：曲洪澜 王树叶[2] 曹峰林[2] 段丽祥[2] 

机构地区：[1]内蒙古林业总医院血液科,内蒙古牙克石市022150 [2]哈尔滨医科大学第一附属医院血液科

出　　处：《临床血液学杂志》2009年第5期469-472,共4页Journal of Clinical Hematology

摘　　要：目的:探讨急性早幼粒细胞白血病(APL)细胞谷胱甘肽S转移酶(GSTπ)表达及临床意义。方法:以患者的外周血或骨髓为研究对象,以正常人的外周血为对照组,用RT-PCR方法检测GSTπ的表达。结果:APL细胞的GSTπ表达36.7%;复发和耐药的APL的GSTπ为60%,复发或耐药组表达较初治组GSTπ表达明显增高(P<0.05),GSTπ表达较高者临床缓解率低(P<0.05)。结论:RT-PCR是有效的特异的敏感的检测方法,As2O3是治疗APL最有效的药物,As2O3可能会下调GSTπ的表达。Objective：To study the methods of quantifyting glutathione S-transferase（GSTπ）gene expression in acute promyelocytic leukemia and the clinical significane. Method： Glutathione S-transferase（GSTn）gene expression in 30 acute promyelocytic leukemia and peripheral blood of 10 healthy persons had been detected by reverse transcription-polymerase chain reaction （RT-PCR）. Result：The expression of GST n in relapse APL patients was higher （P〈0.05 or 〈0. 005）. CR rate in the GST n positive group was lower than that in the GSTπ negative group （P〈0.05）. Conclusion：Our results indicate that reverse transcription-polymerase chain reaction （RT-PCR） is rapid, special and reliable method for detecting GSTn, and GSTπ gene might be a sensitive target for drug resistance, As2O3 is an effective drug of acute promyelocytic leukemia through down-regulating the expression of GSTπ.

关 键 词：白血病 早幼粒细胞性 急性 谷胱甘肽S转移酶 临床意义 

分 类 号：R733.71[医药卫生—肿瘤]