淫羊藿提取物IC163对K562白血病细胞增殖抑制和凋亡诱导效应观察  

The effect of IC163 on inhibiting the growth of K562 and inducing apoptosis

作　　者：朱剑锋[1,2] 赵娜[3] 孟坤 张广森[1] 

机构地区：[1]中南大学湘雅二医院血液科,长沙410011 [2]泰州市人民医院血液科,江苏泰州225300 [3]山东省立医院血液科 [4]北京珅奥基医药科技有限公司

出　　处：《临床血液学杂志》2009年第5期485-487,共3页Journal of Clinical Hematology

摘　　要：目的:观察淫羊藿提取物IC163对K562白血病细胞是否具有增殖抑制和诱导凋亡作用。方法:用MTT实验观察不同浓度的IC163对K562细胞增殖抑制率的影响,用Hochest33258染色法和Annexin V-FITC和PI染色法检测凋亡细胞的百分率,用Western印迹检测药物干预下的K562细胞Caspase-3蛋白表达。结果:IC163以浓度依赖性方式有效地抑制K562细胞增殖,其抑制K562细胞增殖的IC50为18.1μmol/L;IC163以浓度依赖性方式诱导K562细胞凋亡并伴有Caspase-3蛋白表达上调和裂解激活。结论:IC163能够以浓度依赖性方式有效地抑制K562细胞增殖并诱导K562细胞凋亡,其诱导K562细胞的机制可能与Caspase-3凋亡信号启动有关。Objective：To determine the effects of growth inhibition and apoptosis induction of IC163 on K562 leukemia cells. Method：K562 cells were treated with different concentrations of IC163. The cell viability were analyzed with MTT assay, the percentage of apoptosis cells was analyzed by Hoehest33258 staining and Annexin V/ PI via flow cytometric. Result..IC163 can significantly inhibit the proliferation of K562 cells in a concentration--dependent manner, and the IC50 was 18.1 μmol/L; IC163 can induce the apoptosis of K562 cells with an up-regulated Caspase-3 expression and cleavage actation. Conclusion： IC163 could efficiently inhibit the proliferation of K562 cells and induce the apoptosis of K562 cells. So IC163 maybe a potencial anti-leukemia drug.

关 键 词：白血病 IC163 K562 凋亡 CASPASE-3 

分 类 号：R733.7[医药卫生—肿瘤]