大鼠脑出血血肿周围神经元血管内皮生长因子表达与胀亡的初步观察  

The Realtion Between the Expression of VEGF and the Oncosis of Neuron After ICH of Rat

作　　者：刘娜[1] 王淑荣[2] 王连堃[2] 刘永泉[2] 孔祥丽[2] 袁玉英[1] 周勇[1] 

机构地区：[1]齐齐哈尔医学院第五附属医院大庆龙南医院神经内科,黑龙江大庆163453 [2]哈尔滨医科大学附属第四医院神经内科

出　　处：《中国微循环》2009年第4期246-249,F0003,共5页Journal of Chinese Microcirculation

基　　金：国家自然科学基金资助项目(30670727)

摘　　要：目的通过建立大鼠脑出血动物模型观察血管内皮生长因子(VEGF)在出血灶周的细胞定位和动态表达变化和脑出血后神经细胞胀亡时程表达以探讨两者之间的相关性。方法采用二步法制备大鼠脑出血模型,脑出血组注入自体血50μl,对照组注入等量生理盐水,分别于6、24h、2、3、5、7d后处死,行HE染色和免疫组化染色观察脑内VEGF表达变化;电镜下观察胀亡细胞形态学变化并计数。结果脑出血后VEGF的表达与胀亡细胞表达呈负相关。结论VEGF参与了脑出血后的损伤修复。Objective To investigate the interrelationship between the expression of VEGF and the oncosis of neuron after ICH by establishing the rat ICH model through the expression of oneosis of neuron after ICH and the cell location of VEGF-positive cells in perihematoma and the expression of VEGF. Methods The rat ICH model was induced by two-step. ICH was induced by the injection of 50 ul autologous blood into the right caudate nucleus, while 50μl natural saline was injected in the control group. The rats were killed after 6h, 24h, 2d, 3d, 5d and 7d respectively, the morphology changes of nerve cell oncosis and count by electron microscope and the immunohistochemical method and computer image analysis were performed in rats brain by using antibody of VEGF. Results The expression of VEGF has a negative correlation with oncosis, and oncosis has a positive correlation with neurological behaviour. Conclusion VEGF may participate in the recovery after ICH.

关 键 词：脑出血 动物模型 血管内皮生长因子 胀亡 

分 类 号：R364.13[医药卫生—病理学]