白杨黄素对人胃癌细胞SGC7901的放疗增敏作用及其相关机制研究  

Effects of Chrysin on radiation sensitization in human gastric cancer cells SGC7901

作　　者：张强[1] 王红建[1] 王小银[1] 朱金水[1] 达炜[1] 孙群[1] 郭花[1] 

机构地区：[1]上海交通大学附属第六人民医院消化科,上海200233

出　　处：《现代肿瘤医学》2009年第9期1633-1636,共4页Journal of Modern Oncology

摘　　要：目的:探讨白杨黄素对人胃癌SGC7901细胞增殖的放射增敏作用及其相关机制。方法:应用克隆形成实验分别检测不同浓度白杨黄素及60Co γ射线联合应用对人胃癌细胞系SGC7901细胞生存率的影响;应用吖啶橙(AO)/溴化乙碇(EB)荧光染色和TUNEL法分别检测白杨黄素与60Co γ射线联合应用后对人胃癌细胞系SGC7901细胞凋亡的影响;应用Westen-blot检测与细胞凋亡调控相关的蛋白Caspase-3、Survivin和Bcl-2的表达情况。结果:白杨黄素能够明显增强60Coγ射线对人胃癌SGC7901细胞克隆集落形成的抑制作用;增强60Co γ射线导致的人胃癌SGC7901细胞的凋亡;白杨黄素能够上调60Co γ射线处理过后人胃癌SGC7901细胞内活性Caspase的表达,下调细胞内凋亡抑制蛋白Survivin和Bcl-2的表达。结论:对于胃癌细胞系SGC7901,白杨黄素可以作为一种有效的放射增敏剂。Objective：To explore the effects of Chrysin on radiation sensitization in gastric cancer cells , and to elucidate the molecular biology mechanisms. Methods ：The effect of various Chrysin combined with ^60Coγ rays on cell survival rate in human gastric cancer cells was detected by clony formation assay. The influence of Chrysin combined with ^60Coγ rays on inducing apoptosis of SGC7901 were determined by acridine orange （AO） / ethidium bromide （EB） fluorescence staining and TUNEL method. The expression of Caspase - 3, Survivin and Bcl - 2 genes was determined by Westen -blot. Results：The inhibitive effect of ^60Coγ rays on SGC7901 cells clone clony was enhanced by Chrysin. Chrysin can promote the anti - proliferative effect of ^60Coγ rays on SGC7901 cells and up - regulate the expression of Caspase - 3, down - regulate the expression of Survivin and Bcl - 2. Conclusion： Chrysin is an effective radiosensitizer in gastric cancer cell.

关 键 词：胃癌 白杨黄素 放疗增敏 凋亡 

分 类 号：R73-36[医药卫生—肿瘤] R735.2[医药卫生—临床医学]