胶滴肿瘤药敏检测技术在化疗中的研究进展  

Advances of chemotherapy in individual on collagen gel droplet culture drug-sensitivity test

作　　者：郭宁[1] 巩平[2] 

机构地区：[1]石河子大学医学院 [2]石河子大学医学院第一附属医院肿瘤内科,新疆石河子832002

出　　处：《现代肿瘤医学》2009年第9期1807-1809,共3页Journal of Modern Oncology

摘　　要：胶滴肿瘤药敏检测技术(CD-DST)是一项先进、可靠的技术,该方法主要包括原代肿瘤细胞悬液的制备、肿瘤细胞在胶滴内培养、药物处理、无血清培养和图像分析五部分。其特点是原代培养成功率高、通过图像分析软件可以排除成纤维细胞的干扰、临床相关性好,尤其少量标本即可检测的特点,扩大了临床应用范围,使肿瘤个体化治疗成为可能。Collagen gel droplet culture drug- sensitivity test（ CD -DST） is an advanced and reliable technology , this method consists of preparation of primary tumor cell suspension, collagen gel droplet embedded culture step, exposure anticancer drug, a serum - free culture step, and evaluation of anticancer effect by image analysis. This method has many advantages including a high success rate for primary culture, easy quantification of the anticancer effects without contamination with fibroblasts by using an image analysis system. There was a statistically correlation between clinically reported response rates and that obtained by CD - DST, the need for only a small number of cells for the test,Expanded the scope of the clinical application.

关 键 词：肿瘤药敏检测 胶滴肿瘤药敏检测 化疗 

分 类 号：R730.43[医药卫生—肿瘤]