结直肠腺癌CXCR4 CXCL12 Fascin和E-cadherin表达与淋巴结转移的相关性研究  

Correlative Studies on Expression of CXCR4,CXCR12,Fascin and E-cadherin in Colorectal Carcinoma with Lymphatic Metastasis

作　　者：庞秋华[1] 张祥宏[1] 张杰英[1] 李月红[1] 吴文新[1] 王恒树[1] 崔爱荣[1] 张颖[1] 

机构地区：[1]河北医科大学附属第二医院病理科,石家庄市050000

出　　处：《中国肿瘤临床》2009年第16期926-929,933,共5页Chinese Journal of Clinical Oncology

基　　金：河北省自然科学基金资助(编号:C2006000911)~~

摘　　要：目的:探讨CXCR4、CXCL12、Fascin及E-cadherin在结直肠腺-(CRC)中表达及其与淋巴结转移的相关性方法:采用免疫组织化学方法检测126例CRG组织CXCR4、CXCL12、Fascin和E-cadherin在蛋白水平上的表达情况,同时对57例伴有局部淋巴结转移病例的原发癌和其相应淋巴结转移癌上述标志物的表达情况进行对比分析,探讨其表达与淋巴结转移的相关性结果:CXCR4、CXCL12和Fasein在结直肠腺癌中的阳性表达率分别为41.27%、81.75%和47.62%,均明显高于正常结直肠黏膜组织(15.00%、35.00%和15.00%,P均<0.05)。转移组病例Fasein和CXCR4蛋白阳性表达率明显高于未转移组病例(57.89% vs 39.13%和50.88% vs 33.33%,P<0.05)。E-cad-herin在结直肠腺癌组织中阳性表达率明显低于正常黏膜组织( 17.46% vs 80.00%,P<0.05)转移组结直肠癌组织E-cadherin阳性表达率明显低于无转移组(8.77% vs 23.19%,P<0.05)。结直肠腺癌CXCR4阳性表达与肿瘤浸润深度及临床分期密切相关(P<0.05),Fascin阳性表达与肿瘤组织学分级及临床分期均密切相关(P<0.05),而E-cadherin阳性表达与肿瘤组织学分级密切相关(P<0.05)。淋巴结转移癌组织CXCR4、CXCL12和Fascin表达均显著高于其相应原发癌(P<0.05)结论:CXCR4、CXCL 12。Objective： To investigate the correlation between expression of CXCR4, CXCR12, Fascin and E-cadherin and lymph node metastasis in colorectal carcinoma （CRC）. Methods： We used immunohistochem- istry to detect the expression of fascin, CXCR4, CXCL12 and E-cadherin at protein level in 126 cases of CRC and 57 cases of lymph node tissue with metastasis from CRC. Results： Inmmunohistochemical results showed that the positive expression rates of fascin, CXCR4 and CXCL12 in CRC were 47.62%, 41.27% and 81.75%, respectively, which were all significantly higher than those in normal colorectal mucosa （15.00%, 15.00% and 35.00%, P〈0.05）. E-cadherin expression in normal colorectal mucosa was significantly higher than that in CRC tissues （80.00% vs 17.46%, P〈0.05）. The positive expression rates of fascin （57.89%） and CXCR4 （50.88%） in the CRC cases with lymph node metastasis were significantly higher than those in the cases without lymph node metastasis （39.13% and 33.33% respectively, P〈0.05）. Increase in the expression of CXCR4 was positively correlated with depth of invasion and clinical stage in CRC cases （P〈0.05）. Positive expression of fascin in CRC tissues was increased as pathological differentiation and clinical stage increased （P〈0.05）. Positive expression of E-cadherin was negatively correlated with pathological differentiation （P〈 0.05）. Positive expressions of CXCR4, CXCL12, fascin and E-cadherin were not correlated with other clinicopathologic features in CRC （P〉0.05）. The positive expressions of CXCR4, CXCL12 and fascin in lymph nodetissues with metastasis from CRC was significantly higher than those in their corresponding primary carcinoma （P〈0.05）. Conclusion： Changes in the expression of CXCR4, CXCL12, fascin and E-cadherin and their interaction may play important roles in the metastasis of colorectal carcinoma.

关 键 词：结直肠癌Fascin E-cadhefin CXCR4 CXCL12 侵袭转移 免疫组织化学 

分 类 号：R735.3[医药卫生—肿瘤]