葡萄糖转运蛋白4基因多态性与诱导性高血脂症小鼠血脂性状的相关性分析  

Analysis of the relationship between Glut-4 gene polymorphism and blood-fat traits in induced hyperglycemia mice

作　　者：陈琴[1] 王文君[1] 上官新晨[1] 徐明生[1] 

机构地区：[1]江西农业大学食品科学与工程学院,江西南昌330045

出　　处：《药学学报》2009年第8期931-934,共4页Acta Pharmaceutica Sinica

基　　金：国家科技部星火项目(2002EA730001);江西农业大学博士启动基金(2004);江西农业大学自然科学基金(2228);江西省研究生创新专项资助项目(YC08B022)

摘　　要：营养基因组学应用基因组学技术阐明与营养相关的单核苷酸多态性（SNPs），并用来研究人体对某些疾病易感性以及对营养素（食物）需求的个体间差异。通过对营养与基因的相互作用的研究，加深对慢性疾病的更深层次认识。进一步研究表明，个体遗传差异使得部分个体对引起高脂血症的某些环境因素（如饮食）更加易感，高脂血症的发生是营养素与基因互作的结果，膳食对血脂相关基因表达的影响，可从基因转录到翻译后的修饰，人们对这些过程中的细节还不清划引。This study is to investigate the effect of diet and gene on blood-fat trait of the individual mouse. One hundred and twenty mice were fed with high fat emulsion for 4 weeks. Then the genotypes of Glut-4 were analyzed by PCR-SSCP to investigate the effect on blood-fat traits and some organ performance of mice. After fed with high fat emulsion for 4 weeks, 98 mice suffered from hyperlipidemia, while 22 mice did not. And a T/A mutation was found in 188 targeted fragments we amplified at 8 521 site, and association analysis showed that the mice carried BB genotype had higher TC, TG and LW/BW level than those of the mice with AA genotype （P 〈 0.05）. Different Glut-4 genotypes show different sensitivities to high fat emulsion on mice.

关 键 词：Glut-4基因 多态性 高血脂症小鼠 血脂性状 

分 类 号：R963[医药卫生—微生物与生化药学]