ELISA法检测肿瘤相关抗原神经元特异性烯醇化酶的方法学评价  

Methodological Evaluation of ELISA Assay for Neuron Specific Enolase

作　　者：郭广宏[1] 王建柱[1] 丁慧[1] 董振南[1] 金剑 

机构地区：[1]解放军总医院生化科,北京100853 [2]瑞典康乃格诊断公司,北京100022

出　　处：《标记免疫分析与临床》2009年第4期247-249,共3页Labeled Immunoassays and Clinical Medicine

摘　　要：对肿瘤相关抗原神经元特异性烯醇化酶(NSE)ELISA检测方法进行综合评价。选择临床诊断为小细胞肺癌、非小细胞肺癌及神经母细胞瘤等肿瘤患者、其它非肺癌肿瘤患者、良性疾病患者及健康人样本共223例,进行盲法比较。本方法的灵敏度为60.2%,特异性为93.8%,阳性预测值为87.5%,阴性预测值为76.7%。NSE在各入选人群中的阳性率分别为:小细胞肺癌组84.6%,非小细胞肺癌组47.6%,肺癌组总阳性率为58.4%,神经母细胞瘤组阳性率为100%,非肺癌肿瘤组的阳性率为13.3%,良性疾患组阳性率为0%,健康人群组阳性率为0%。中国正常人群cut-off值为7.78μg/L,与试剂盒提供的cut-off值相似。NSE在小细胞肺癌及神经母细胞瘤患者中有很高的阳性率,尤其是对于神经母细胞瘤有很高的临床意义。NSE在正常健康人及良性疾病患者、非肺癌肿瘤患者中的阳性率较低,检查NSE有助于肿瘤的鉴别诊断。To evaluate the methodology of ELISA for neuron specific enolase（NSE）,223 serum samples which include patients with SCLC,NSCLC,neuroblastoma,non-lung cancer,benign diseases and healthy people were selected.The sensitivity of this method was 60.2%,specificity was 93.8%,PPV was 87.5% and NPV was 76.7%.In different group of patients with SCLC,NSCLC,neuroblastoma,non-lung cancer,benign diseases and healthy people,the positive rate of NSE were 84.6%,47.6%,100%,13.3%,0% and 0% respectively.The cut-off value was 7.78 μg/L in Chinese healthy population.NSE was significant in SCLC and neuroblastoma.NSE is a very useful tumor marker in clinical diagnosis of cancer.

关 键 词：神经元特异性烯醇化酶 小细胞肺癌 非小细胞肺癌 酶联免疫吸附法 CUT-OFF值 

分 类 号：R730.4[医药卫生—肿瘤]