酶标仪测定戊二醛溶液含量  

Glutaral Solution Conternts Determined by Enzyme-labeled Device

作　　者：肖雨清[1] 肖小波 胡海霞[3] 曹玫[1] 尤世刚[1] 肖萍[1] 

机构地区：[1]郧阳医学院附属人民医院药学部,湖北十堰442000 [2]湖北省沙洋县第二人民医院检验科,湖北荆门448272 [3]郧阳医学院药学系,湖北十堰442000

出　　处：《中华医院感染学杂志》2009年第17期2288-2290,共3页Chinese Journal of Nosocomiology

基　　金：湖北省卫生厅科研基金(JX2C18)

摘　　要：目的探讨以酶标仪一次完成多样品测定的戊二醛溶液含量快速分析方法。方法取戊二醛对照溶液及样品溶液测定光密度值,通过与已知浓度戊二醛比较,计算样品含量。结果戊二醛含量在0.75%～2.50%,浓度与光密度存在线性关系;重复性试验:相对平均误差<2%,相对标准偏差≤2.51%;干扰试验,抗氧剂亚硝酸钠可使检验结果出现负误差,0.1%亚硝酸钠可使检验结果相对下降1.5%。结论酶标仪群测使用中戊二醛含量方法简单,方便快捷,准确性符合院感监测要求,添加剂对检测的干扰可接受,具有很强实用性。OBJECTIVE To study the analytic method for assaying of glutaral concentration by enzyme-labeled device at one time. METHODS Controlled solution of glutaral and sample solution were taken out and kept until the room temperature. Optical density value was detected at 450 nm. Content of sample was calculated by comparison with the known concentration of glutaral. RESULTS In the range of 0. 75-2. 50%, concentration of glutaral had linear relationship with the optical density value. Reproductive test： relative average error was within 2M, RSD≤.2. 51%; interference test： oxidation-resistant-sodium nitrite could make the result negative error. 0.1% Sodium nitrite could make the test result decrease by 1. 5% relatively. CONCLUSIONS The method is simple, fast and convenient, with accuracy which has met the requirement of hospital infection monitoring and acceptable interfering range made by additives, which is highly practical.

关 键 词：酶标仪 斐林试剂 戊二醛 含量测定 

分 类 号：R187[医药卫生—流行病学]