反相高效液相色谱法测定马铃薯茎叶废弃物中的茄尼醇含量  

Determination of solanesol in waste potato leaves and stems by RP-HPLC

作　　者：马君义[1] 张继[1] 柳国学[1] 陈桐[1] 徐小龙[1] 

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《西北师范大学学报（自然科学版）》2009年第4期72-75,共4页Journal of Northwest Normal University(Natural Science)

基　　金：高等学校科技创新工程重大项目培育资金项目(706055);甘肃省科技攻关计划项目(2GS064-A41-004)

摘　　要：以石油醚为溶媒,采用索氏提取法提取马铃薯（Solanlum tuberosum L.）茎叶废弃物中的茄尼醇,并用反相高效液相色谱法测定其含量.结果表明,马铃薯废弃叶中茄尼醇平均含量为413.2μg.g^-1,废弃茎中茄尼醇平均含量为30.9μg.g^-1,皂化能使结合态的茄尼醇游离出来,从而提高茄尼醇的含量.该方法的线性范围为3.125-150.000μg.mL^-1,精密度检测的RSD为1.32%,重复性试验的RSD为1.63%,平均回收率为96.56%.Petroleum ether is used as extraction solvent in soxhlet extraction method to extract solanesol from waste potato leaves and stems. The content of solanesol is determined by the reversed phase high performance liquid chromatography method （RP-HPLC）. There are about 413.2 μg·g^-1 and 30. 9 μg·g^-1 of solanesol in waste potato leaves and stems respectively. Saponification can improve the content of solanesol in the extractions. The linearity range of the proposed method is from 3. 125 - 150. 000 μg· mL^-1, the precision test is 1.32%（RSD）, the repetition test is 1.63% （RSD）, and the average recovery rate is 96.56%.

关 键 词：马铃薯 茄尼醇 反相高效液相色谱法 索氏提取 

分 类 号：Q946[生物学—植物学]