Glu-1位点等位变异及表达量对麦谷蛋白聚合体粒度分布的影响  

Effect of Allelic Variation and Expression Quantity at Glu-1 Loci on Size Distribution of Glutenin Polymer in Common Wheat

作　　者：张平平[1] 马鸿翔[1] 姚金保[1] 何中虎[2,3] 

机构地区：[1]江苏省农业科学院农业生物技术研究所,江苏南京210014 [2]中国农业科学院作物科学研究所/国家小麦改良中心/国家农作物基因资源与基因改良重大科学工程,北京100081 [3]国际玉米小麦改良中心中国办事处,北京100081

出　　处：《作物学报》2009年第9期1606-1612,共7页Acta Agronomica Sinica

基　　金：引进国际先进农业科学技术(948计划)项目(2006-G2);江苏省博士后科研资助计划项目(0702011C)资助

摘　　要：选用我国春播麦区23份(试验I)和北部冬麦区21份(试验II)品种(系),研究了Glu-1位点等位变异及其亚基表达量对谷蛋白聚合体粒度分布的影响。结果表明,Glu-1位点等位变异及其亚基表达量显著影响谷蛋白聚合体的粒度分布,且影响程度受蛋白质含量,尤其是高分子量谷蛋白总量水平的影响。在高分子量谷蛋白总量较低时(试验I),Glu-B1和Glu-D1位点对不溶性谷蛋白大聚体含量(UPP)及其占聚合体蛋白总量的百分比(%UPP)的加性效应都达1%显著水平;Glu-B1和Glu-D1位点单个亚基对两者的贡献分别为7OE+8*>7+9>17+18>7+8和5+10>2+12,具有5+10亚基组合的%UPP显著高于具有2+12的亚基组合。高分子量谷蛋白的亚基表达量与UPP含量呈高度正相关,相关系数为0.79～0.93(P<0.01)。而在高分子量谷蛋白总量较高时(试验II),仅Glu-D1位点对%UPP的加性效应达5%显著水平,5+10亚基对%UPP的贡献显著高于2+12和4+12;亚基组合间的聚合体粒度分布无显著差异。高分子量谷蛋白的亚基表达量与UPP含量的相关系数为0.42～0.86(P<0.05或0.01)。结合高分子量谷蛋白表达量和优质亚基进行选择,能有效提高不溶性谷蛋白大聚体的含量和相对比例,有利于面筋强度和加工品质的进一步提高。The size distribution of glutenin polymers is a key factor in determining gluten strength and end-use quality, while the relationship with the quality and quantity of subunits at Glu-1 loci has been not studied in detailed. Twenty-three spring genotypes （Trial Ⅰ） and twenty-one winter genotypes （Trial Ⅱ） were used to study the effect of allelic variation and expression quantity at the Glu-1 loci on the size distribution of glutenin polymers. The results showed that the size distribution of glutenin polymers was significantly affected by allelic variation and subunits in expression quantity at the Glu-1 loci based on flour protein content, especially on total expressions of high-molecular-weight glutenin subunits （HMW-GS）. In Trial Ⅰ, low expression of HMW-GS was presented, significantly additive effects were observed at Glu-B1 and Glu-D1 loci （P 〈 0.01） for SDS-unextractable polymeric protein （UPP） and percent SDS-unextractable polymeric protein in total polymeric protein （%UPP）. The contribution of individual glutenin subunit could be ranked as 7^OE＋8^＊ 〉 7＋9 〉 17＋18 〉 7＋8 and 5＋10 〉 2＋12 at Glu-B1 and Glu-D1 loci, respectively. Higher %UPP was observed in those allelic compositions with subunit 5＋10 than with 2＋12. The expression of HMW-GS was highly positively correlated with UPP （r = 0.79-0.93）. While in Trial Ⅱ, high expression of HMW-GS was presented, significantly additive effects were only observed at Glu-D1 loci （P 〈 0.05） for %UPE The contribution of individual glutenin subunit ranked as 5＋10 〉 2＋12 and 4＋12 at Glu-D1 loci for %UPP, and no significant difference was observed among allelic compositions for the size distribution of polymers. The expression of HMW-GS was positively correlated with UPP （r = 0.42-0.86, P 〈 0.05 or 0.01）. In conclusion, gluten strength and end-use quality can be improved by selection of high quality subunits in combination with high expression in breeding program.

关 键 词：普通小麦 Glu—1位点 谷蛋白聚合体 粒度分布 

分 类 号：S512.1[农业科学—作物学]