解冻温度对大额牛(Bos frontalis)精子活率及形态的影响  

Effect of Thawing Temperature on Live Sperm Count and Morphological Profile of Gayal (Bos frontalis) Semen under Normal Temperature

作　　者：和占星[1] 赵家才 黄必志[1] 袁希平[1] 

机构地区：[1]云南省草地动物科学研究院,云南昆明650212 [2]云南省家畜冷冻精液站,云南昆明650212

出　　处：《西南农业学报》2009年第4期1093-1098,共6页Southwest China Journal of Agricultural Sciences

基　　金：云南省应用基础研究计划面上项目(2006C0068M);云南省草地动物科学研究院院长基金项目(YNBP2006DF002);云南省科技攻关与高新技术发展项目(2006HG23)

摘　　要：分别用32、35、38、41和44℃水浴解冻大额牛细管冻精,解冻后在常温(平均温度18.06±1.50℃,相对湿度46.64±4.52%)下保存40h,分别在0、6、12、18、24、30、36、38、39和40h取样在显微镜下观察记录精子活率和活力;并在解冻后0、6、12、18和24h用姬姆萨染色,在显微镜下观察记录精子形态,统计分析5个时段的精子畸形率。研究结果:①35℃解冻的精子复苏率为68.80%,极显著高于44℃解冻的54.2%(P<0.01),但与其它3个解冻温度比较差异不显著(P>0.05)。②以38～44℃解冻的精子存活时间在38～39h之间,以32℃解冻的存活时间最短,仅为36h。④解冻后0h的精子活率在42.50%～50.00%之间,不同解冻温度间差异不显著(P>0.05);保存6、12和18h时,分别以35、38和41℃显著高于44℃(P<0.05);保存24h后,各解冻温度间差异不显著P>0.05)。④解冻后0h的精子活力以35℃极显著高于44℃(P<0.01);保存6h以32～41℃显著高于44℃(P<0.05);保存18h,以32和41℃高于44℃(P<0.05);保存24h后各解冻温度间差异不显著(P>0.05)。⑤用41和44℃解冻的总精子畸形率极显著高于38℃(P<0.01),显著高于35℃(P<0.05),32、35和38℃间及32、41和44℃间差异不显著(P>0.05),用35和38℃解冻对精子形态损伤较小。研究表明:解冻温度越高大额牛冻精的精子活率和活力降低速度越快,精子畸形率也高,且解冻温度对精子头部和尾部形态的影响最大;大额牛细管冻精宜用38±3℃水浴10s解冻,解冻后在18℃以下常温下保存6h对其精子活力等影响不大。The objective of paper was to provide the practical thaw temperature of gnyal（ Bos frontalis ） frozen semen in order to improve utilization rate of frozen semen and pregnancy rate of artificial insemination （ AI ） of rare gayal with semi-wildness through careful study of thaw temperature on the live spermatozoa count（ % ）, livable time, progressive motility（ % ） and morphological profile of gayal thawing frozen semen under room temperature. Straw frozen semen of gayal was thawed at 32, 35, 38, 41 and 44 ℃ by a water bath. And post-thaw straw semen was left in room temperature （average temperature and relative humidity recorded during the experiment under 18.06 ± 1.50 ℃ and 46.64 % ±4.52 %, respectively） for40 h, and especially live sperm count and progressive motility of gayal straw semen were observed and counted at 0, 6, 12, 18, 24, 30, 36, 38, 39 and 40 h, respectively. Meanwhile, morphological profile of gayal semen with Giemsa staining was observed from various fields under compound microscope at 400 × magnification and the number of normal and abnormal were counted at 0, 6, 12, 18, 24 h and appropriately expressed as pereentage value. The results were showed that （i） Resuscitate rate of thaw semen at 35 ℃ was 68.8 %, and significantly higher than that at 44 ℃ Livable time （ 54.2 % ） （ P 〈 0.01 ）, and not significantly higher than those of other three thaw temperature（ P 〉 0.05 ）. （ii） Livable time of thawed semen at 38 -44 ℃ lasted for 38 -39 h, and thawed semen were completely dead at 40 h. Moreover, livable time of thawed semen at 32 ℃ lasted hardly for 36h. （iii） The live spermatozoa count of thawed semen at Oh was 42.50 % - 50.00 %, and there was not a significant difference among different thawed temperature（ P 〉0.05） ; The live sperm count of thaw spermatozoa at 35, 38 and41 ℃ left in room temperature for6, 12 and 18 h were in turn 45 %, 40 % and 16.3 %, and significant higher than those at44 ℃ （P〈0.05）, and there was not a

关 键 词：大额牛 精子 解冻温度 活力 畸形 

分 类 号：S823[农业科学—畜牧学]