PTEN和p53蛋白异常表达与子宫内膜异位症恶变的关系  

Correlation of the protein expression of PTEN and p53 with malignant transformation of endometriosis

作　　者：马小平[1] 甄宗慧[2] 徐冰[1] 戴淑真[1] 赵鹏[3] 魏志敏[3] 

机构地区：[1]青岛大学医学院附属医院妇科,山东青岛266003 [2]滕州市工人医院妇产科,山东滕州277500 [3]青岛大学医学院附属医院病理科,山东青岛266003

出　　处：《泰山医学院学报》2009年第4期241-244,共4页Journal of Taishan Medical College

基　　金：山东省科技攻关计划(项目编号2006GG2202027)

摘　　要：目的探讨PTEN基因和p53基因蛋白表达异常与子宫内膜异位症(简称内异症)恶变的关系以及两者在其发生发展过程中的相互作用。方法应用免疫组化PV-9000二步法检测23例内异症相关卵巢癌(EAOC)、10例不典型内异症(aEMs)和20例典型卵巢内异症(EMT)组织中PTEN和p53基因蛋白表达情况。结果EAOC中PTEN、p53基因蛋白表达缺失率分别为78.26%和17.39%,前者高于EMT组(5%)和aEMs组(20%),后者低于EMT组(85%)和aEMs组(70%)。EAOC中PTEN、p53基因蛋白表达缺失率与EMT组相比差异有高度统计学意义(P<0.01),与aEMs组相比差异有统计学意义(P<0.05)。EAOC中PTEN、p53基因蛋白表达缺失率均与临床分期、病理分级、是否伴有转移密切相关(P<0.05),与年龄、组织学类型无关(P>0.05),不同的是,PTEN在临床分期早、组织分化好、无转移的EAOC中的蛋白表达缺失率明显低于临床分期晚、组织分化差和(或)伴转移者,而p53蛋白表达相反。PTEN和p53基因的蛋白表达呈负相关。结论PTEN蛋白表达缺失和p53蛋白过表达与内异症相关卵巢癌及内异症恶变的发生发展密切相关。Objective： To investigate the relationship between protein expressions of PTEN and p53 genes and malignant transformation of endometriosis. Methods： Twenty-three endometriosis associated ovarian cancers （EAOC）, 10 atypical hyperplasia endometriosis of ovary and 20 typical endometriosis tissues were used to detect the protein expressions of PTEN and p53 genes using immunohistochemistry PV-9000 method. Among the twenty-three EAOC samples, nine were endometrioid carcinomas, fourteen were clear cell carcinomas, and four of them demonstrated continuity between cancer and endometriosis tissue. Results： Eighteen of the twenty-three EAOC cases （78.26%） and four （ 17.39% ） had PTEN and p53 protein expression deletion, with the deletion rates of PTEN higher and p53 lower than those of EMT and aEMs respectively. The protein expression deletion rates of PTEN in EMT and aEMs were 5% and 20%, but those of p53 were 85% and 70% respectively. There was significantly difference when EAOC was compared with EMT or aEMs （ P 〈 0.05 ）. The rates of protein expression of PTEN/p53 of EAOC were not relative to age difference and histological type （ P 〉 0.05 ）, and but had significant relationship with clinical stage, pathological grade or accompanied metastasis （ P 〈0.05 ）. The rate of protein expression deletion of PTEN of EAOC which was in early clinical stage, well-differentiated and not accompanied by metastasis was obviously lower than that which was in late clinical stage, poor-differentiated and accompanied metastasis. However, there was an opposite expression of p53. Obviously, it was negtively correlated with the protein expression of PTEN and p53. Conclusion： There is intimate correlation between aberrant expressions of PTEN and p53 genes and malignant transformation of endometriosis.

关 键 词：子宫内膜异位症 卵巢肿瘤 不典型增生 PTEN基因 P53基因 免疫组化 

分 类 号：R711.71[医药卫生—妇产科学]