鸡Mx基因编码序列1892位SNP及其等位基因的确定  

Identification of SNP at nucleotide position 1892 of coding sequence of the chicken Mx gene

作　　者：金丽娜[1] 韩建林[1,2] 罗玉柱[1] 

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730070 [2]中国农业科学院国际家畜研究所畜禽及牧草遗传资源联合实验室,北京100094

出　　处：《甘肃农业大学学报》2009年第4期32-35,共4页Journal of Gansu Agricultural University

基　　金："十一五"国家科技支撑计划项目(2006BAD13B08);中国农业科学院杰出人才一级岗位基金项目

摘　　要：通过利用非互补的PCR-RFLP技术检测了12个鸡群体378个样本在Mx基因编码序列第1892位的等位基因.结果表明:鸡Mx基因经PCR-RFLP扩增,获得长度约为100 bp的基因片段,在1892位发生A→G的突变;鸡Mx的抗病性(A)和易感性(G)等位基因频率分别为38%和62%,且分布于西南亚的BAN、PKD、PMA和Pal 4个鸡群体中的抗病基因频率明显高于其他群体.A G/A SNP at nucleotide position 1,892 of coding sequence of chicken Mx gene （a single amino acid substitution between Asn and Ser at position 631 in the chicken Mx protein） has been previously demonstrated to confer susceptibility/resistance to avian viral diseases. In this study,it was determined the resistant or susceptible Mx allelic gene in 378 samples from 12 chicken populations by using the mismatched PCR-restriction fragment length polymorphism （RFLP） technique. The rates of the resistant Mx gene and susceptible gene were 38 % and 62 %, respectively. And it is very interesting that the frequency of the resistant Mx gene in 4 populations from Southwest Asia （BAN, PKD, PMA and PAL） was obviously higher than the others.

关 键 词：MX 基因 单核苷酸多态性 抗病性 PCR-RFLP 等位基因频率 

分 类 号：S831[农业科学—畜牧学] Q785[农业科学—畜牧兽医]