A new metabonomics method for simultaneous determination of EFAs and NEFAs in plasma using GC-MS and its application  

作　　者：Li Da Han Qiong Lin Liang Yi Ming Wang Ping Hu Guo An Luo 

机构地区：[1]School of Pharmaceutics, East-China University of Science & Technology, Shanghai 200237, China [2]Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China

出　　处：《Chinese Chemical Letters》2009年第9期1103-1106,共4页中国化学快报（英文版）

基　　金：offered by China-Japan Friendship Hospital(Beijing,China);supported by grantfrom the National Basic Research Development Program of China(973 Program,No.2005CB523503);the National Natural Science Foundations of China(Nos.90709045 and 20805026)

摘　　要：A new metabonomics method was developed for simultaneous qualitative and quantitative analysis on esterified and nonesterified fatty acids （EFAs and NEFAs） in plasma. Merely 10 μL of plasma was required. The pretreatment of the sample was simple without disposing the protein. After simple extraction and derivation, 15 FAs in plasma were precisely quantified. Gas chromatography tandem mass spectrometry （GC-MS） was used in the study and the quantities of the analytes, which varied in abundance over three orders of magnitude. The established method showed good accuracy. The curve correlation coefficients were all above 0.997. The RSDs of precision for all compounds were below 15% and recoveries were all between 80 and 110%. PLS-DA modal was used in the data management. 2009 Guo An Luo. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.A new metabonomics method was developed for simultaneous qualitative and quantitative analysis on esterified and nonesterified fatty acids （EFAs and NEFAs） in plasma. Merely 10 μL of plasma was required. The pretreatment of the sample was simple without disposing the protein. After simple extraction and derivation, 15 FAs in plasma were precisely quantified. Gas chromatography tandem mass spectrometry （GC-MS） was used in the study and the quantities of the analytes, which varied in abundance over three orders of magnitude. The established method showed good accuracy. The curve correlation coefficients were all above 0.997. The RSDs of precision for all compounds were below 15% and recoveries were all between 80 and 110%. PLS-DA modal was used in the data management. 2009 Guo An Luo. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.

关 键 词：METABONOMICS Fatty acids Quantification GC-MS PLS-DA 

分 类 号：O657.71[理学—分析化学] S963.21[理学—化学]