雷公藤总生物碱对粘虫中肠细胞超微结构及消化酶活性的影响  

Effects of the total alkaloid from Tripterygium wilfordii Hook on ultrastructure of the midgut cells and the digestive enxyme activities of Mythimna separata(Walker) larvae

作　　者：周琳[1,2] 马志卿[2] 冯俊涛[2] 张兴[2] 

机构地区：[1]河南农业大学植物保护学院,河南郑州450002 [2]西北农林科技大学无公害农药研究服务中心,陕西杨凌712100

出　　处：《河南农业大学学报》2009年第4期418-421,共4页Journal of Henan Agricultural University

基　　金：国家"十五"科技攻关项目(2002BA516A04);河南省科技攻关项目(082102350033)

摘　　要：采用电子显微镜技术与生化分析研究了雷公藤总生物碱对粘虫幼虫中肠组织及主要消化酶的影响.电镜观察结果表明,中毒试虫的中肠细胞及其细胞器发生明显病变,肠壁细胞微绒毛零乱、脱落;线粒体肿胀,出现空白亮区,双层膜不完整;内质网池扩张,囊泡化,粗面内质网减少.消化酶活性测定结果表明,中毒试虫中肠蛋白酶、淀粉酶及脂肪酶的活性和正常试虫相比无明显变化.Electronic telescopic technology and biochemical analysis were used to study the effect of the total alkaloid from Tripterygium wilforclii Hook on uhrastructure of the midgut cells and the digestive enzyme activities of Mythimna separata （Walker） larvae. Observation results of electronic microscope showed midgut cells and their organelles of poisoned M. separata larvae to the total alkaloid from T. wilfordii exhibited distinct pathological changes. The microvilli of midgut cells were disordered and came off markedly. The mitochondria was irregularly enlarged , the bilayer membrane was not intact, and the cisternae of rough endoplasmic reticulum were distended. The results of biochemical analysis showed the activities of protease, amylase and lipase of the poisoned larval midgut had no significant changes compared with that of the control.

关 键 词：雷公藤 总生物碱 粘虫 超微结构 消化酶 

分 类 号：S842.39[农业科学—畜牧兽医]