负载辅酶Q10的两亲性聚肽-壳聚糖复合纳米粒的制备及性质研究  

Preparation and Characterization of Amphiphilic Polypeptide-chitosan Complex Nanoparticles Containing Coenzyme Q10

作　　者：章苏宁[1] 顾翠英[2] 张健[1] 贺振颖[1] 

机构地区：[1]上海应用技术学院香料香精技术与工程学院,上海200235 [2]上海中医药大学医学技术学院,上海201203

出　　处：《化学世界》2009年第9期521-524,517,535,共6页Chemical World

基　　金：上海市教育委员会自然基金资助项目(06OZ005)

摘　　要：以两亲性聚肽-壳聚糖复合体系为壁材,采用自组装及离子凝聚法制备负载辅酶Q10的纳米粒子,探讨了制备纳米粒子的较佳条件,采用高分辨率透射电镜、动态激光散射仪、紫外光谱及Zeta电位分析仪对优化的聚肽-壳聚糖复合体系纳米粒子进行表征,结果表明:聚肽-壳聚糖复合体系纳米粒子是具有壳核结构的球型粒子;纳米粒子粒径大小在80 nm到300 nm之间,粒径分布较均匀,稳定性较好,粒子的载药率为3.46±0.3%,包封率为59.7±2.1%。24 h的累积释放为75%。Biocompatible amphiphilic polypeptide-chitosan was used to prepare nanoparticles containing coenzyme Q10 by self-assembly and ion gel methods. The optimal preparation conditions of polypeptidechitosan complex nanoparticles were investigated. The properties of nanoparticles containing coenzyme Q10 were studied by transmission electron microscopy, dynamic light scattering, ultraviolet-visible spectroscopy and zeta potential analyzer. The results showed that the morphology of the nanoparticles is spherical with a shell-core structure, the size of the nanoparticles is from 80 nm to 300 nm, and the distribution is homogeneous. The particles have good stability. The drug-loading amount of coenzyme Q10 is 3.46±0.3%, the entrapment efficiency is 59.7±2.1%, and the 24 h accumulative in vitro release is 75%.

关 键 词：两亲性聚肽共聚物 壳聚糖 辅酶Q10 纳米粒子 

分 类 号：O636[理学—高分子化学]