比较基因组杂交技术在检测急性淋巴细胞白血病高二倍体中的意义  

Application of comparative genomic hybridization to hyperdiploid acute lymphoblastic leukemia

作　　者：曹琪[1,2] 吴钦武 苏欣莹 牛超[1,2] 何凯莉 陈竺[1,2] 陈赛娟 

机构地区：[1]上海第二医科大学附属瑞金医院 [2]江西上饶县人民医院

出　　处：《中华血液学杂志》1998年第7期363-366,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金;英国Lady Tata Memorial Trust基金

摘　　要：目的：评价比较基因组杂交（ＣＧＨ）技术在白血病研究中应用的价值。方法：应用ＣＧＨ技术检测高二倍体急性淋巴细胞白血病（ＡＬＬ）患者１４例，并与核型分析结果相比较。结果和结论：发现在高二倍体ＡＬＬ中，部分和（或）整条染色体的增加比减少常见，两者相差７倍，其中２１号和Ｘ染色体的增加最为常见。ＣＧＨ与常规Ｒ带的分析结果比较可分为三种：①ＣＧＨ和常规核型分析结果完全一致的有３例；②ＣＧＨ分析和Ｒ带分析从不同角度提供了相对一致信息的有８例；③ＣＧＨ分析和Ｒ带分析结果不一致的有３例，其中２例分别为约三倍体和约四倍体。Objective: To evaluate the implication of comparative genomic hybridization (CGH) in leukemia study. Methods: Genomic abnormalities in 14 ALL patients were assayed by CGH, and the results were compared with those of conventional karyotype analysis. Results and Conclusion: Regional and/or whole chromosome overrepresentation was found to be more frequent than underrepresentation (43 gains versus 6 losses), the most common gains involved being chromosomes 21 and X. Comparison between the results of CGH and conventional Rbanding analysis showed that: ①In 2 cases with trisomy, both the methods gave identical results.②In 8 cases, both the results were consistent excepting for minor discrepancies. ③In 3 cases, including 2 each with triploidy and tetraploidy respectively, and one with chimeric karyotype of normal/+22, the results from the two methods were discrepant.

关 键 词：白血病 ALL 比较基因组杂交 高二倍体 检测 

分 类 号：R733.71[医药卫生—肿瘤]