子宫内膜良恶性病变组织Syk基因表达的初步研究  

Expression of Syk gene in benign and malignant lesions of endometrium

作　　者：陈荣辉[1] 马德花[1] 孙桂霞[1] 赵淑萍[1] 

机构地区：[1]青岛大学医学院附属医院妇科,山东青岛266003

出　　处：《中华肿瘤防治杂志》2009年第16期1250-1252,共3页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:探讨Syk基因在子宫内膜良性及恶性病变中的表达及其在子宫内膜癌发生、发展中的可能作用。方法:分别采用逆转录-聚合酶链反应及蛋白质印迹法检测52例子宫内膜癌、30例子宫内膜不典型增生、28例复杂型增生、30例单纯型增生和40例正常子宫内膜组织中Syk基因及蛋白的表达。结果:从正常子宫内膜组、不典型增生组至子宫内膜癌组中Syk基因和蛋白表达依次降低,差异有统计学意义,P<0.05;Syk基因和蛋白在复杂型增生、单纯型增生及正常子宫内膜中的表达差异无统计学意义,P>0.05;子宫内膜癌中,Syk基因和蛋白的阳性表达与临床分期(P<0.05)、淋巴结转移(P<0.01)以及肌层浸润深度(P<0.05)明显相关,而与组织学分级无明显相关性,P>0.05。结论:Syk低表达或缺失可能与子宫内膜癌的发生、浸润及转移有关。OBJECTIVE：To investigate the expression of Syk gene in benign and malignant lesions of endometrium and investigate the possible role of Syk gene in the oncogenesis and metastasis of endometrial carcinoma. METHODS： By using RT-PCR and Western blot technique, specimens from 52 cases of endometrium carcinoma, 30 atypical hyperplasia endometrium, 28 complex hyperplasia endometrium, 30 simple hyperplasia endometrium, and 40 normal endometrium were detected for their expressions of Syk gene and Syk protein. RESULTS： The positive expressions of Syk gene and Syk protein was gradually increased in endometrial carcinoma tissues, endometrial atypical hyperplasia tissues, endometrial complex hyperplasia tissues, endometrial simple hyperplasia tissues, and normal endometrial tissues （P〈0.05）. However, there was no significant difference among endometrial complex hyperplasia, endometrial simple hyperplasia tissues, and normal endometrial tissues （P〉0.05）. The positive expressions of Syk gene and Syk protein were significantly related to the clinical stage of endometrial carcinoma （P〈0.05）, lymph node metastasis （P〈0. 01）, and the depth of infiltrating muscular layer （P〈0.05）, but not correlated with the histological grade of endometrial carcinoma （ P 〉 0. 05 ）. CONCLUSION ： The lower expression or deletion of Syk gene may play an important role in tumor oneogenesis, infiltration, and metastasis in endometrial carcinoma.

关 键 词：子宫内膜肿瘤 脾酪氨酸激酶 基因表达 逆转录聚合酶链反应 免疫印迹法 

分 类 号：R737.33[医药卫生—肿瘤]