肺癌组织SKP2和p16蛋白表达及其临床意义的探讨  

Clinical significance of expressions of SKP2 and p16 proteins in lung carcinoma tissues

作　　者：赵君[1,2] 丁卫忠[2] 刘景义[2] 王鹏航 柳致平 杨春鹿[1] 

机构地区：[1]中国医科大学附属第一医院胸外科,辽宁沈阳110001 [2]沈阳市胸科医院胸外科,辽宁沈阳110044

出　　处：《中华肿瘤防治杂志》2009年第14期1093-1096,共4页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:研究SKP2和p16蛋白在肺癌组织中的表达,探讨它们之间的关系及其临床意义。方法:利用组织芯片和免疫组化技术检测SKP2和p16蛋白在89例非小细胞肺癌(NSCLC)、13例小细胞肺癌(SCLC)及10例肺良性病变组织中的表达。结果:NSCLC、SCLC及肺良性病变组织中SKP2和p16蛋白表达阳性率分别为(23.52±13.57)%、(44.72±15.97)%,(53.85±12.26)%、(28.25±5.68)%及(2.91±1.27)%、(91.13±6.57)%,三者之间差异有统计学意义,P值均为0.000。SKP2蛋白在肺癌组织中的表达水平与细胞分化程度、病理类型、TNM分期及淋巴转移密切相关,P值均为0.000。p16蛋白在肺癌组织中的表达水平与细胞分化程度,病理类型,TNM分期,淋巴转移密切相关,P值均为0.000。肺癌组织中SKP2和p16蛋白表达呈负相关,r=-0.660,P=0.000;SKP2蛋白表达率与肿瘤的恶性程度呈负相关,r=-0.286,P=0.000;p16蛋白表达率与肿瘤的恶性程度呈正相关,r=0.132,P=0.000。结论:SKP2蛋白表达增高与p16蛋白表达降低共同在肺癌的发生发展中起促进作用,且它们对肿瘤的恶性程度也有相反的影响,但它们之间相互作用的具体机制尚需进一步研究。OBJECTIVE： To explore the expressive character istics of SKP2 and p16 proteins in lung carcinoma tissues and their affection to patients prognosis. METHODS： The expressions of SKP2 and p16 proteins were detected in 89 NSCLC, 13 SCLC, 10 lung benign lesion tissues by tissue chip and immuno-histochemistry technology. RESULTS： The positive rates of SKP2 and p16 protein stain were （23.52 ± 13.57）% and （44.72 ± 15.97）% （53. 85±12. 26） % and （28.25±5.68）%0, （2.91±1.27）% and （91.13±6.57）% in NSCLC, SCLC and lung benign lesion tissues, respectiveely. There were significantly differences among them（P=0. 000）. The expressive level of SKP2 protein in lung carcinoma tissues was closely related to the cell differentiation, pathological type, TNM stage and lymph node metastasis （P=0. 000）. The expressive level of p16 protein in lung carcinoma tissues was closely related to the cell differentiation, pathological type, TNM stage, lymph node metastasis （P=0. 000）. The expression of SKP2 protein was negative correlation with p16 protein in NSCLC（r= - 0. 660, P 0. 000）. The expression rate of SKP2 protein in lung carcinoma was negative correlation with tumorous malignant degree（r=-0.286, P=0.000）. The expression rate of p16 protein in NSCLC was positive correlated with tumorous malignant degree （ r = 0. 132, P = 0. 000）. CONCLUSION ： The high expression of SKP2 protein and low expression of p16 protein concurrently play a facilitative role in the occurrence and development of lung carcinoma. Moreover, there i s a certain opposite role to lung tumorous malignant degree. Yet, the interactive mechanism between them needs further research.

关 键 词：肺肿瘤 S期激酶相关蛋白质类 P16蛋白 组织芯片 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]