互隔交链孢酚激活EC9706细胞DNA聚合酶β表达的信号通路初探  

A preliminary study of signal trusduction of DNA polymerase β expression in EC9706 cells induced by alternariol

作　　者：赵继敏[1] 李沛[1] 杨洪艳[1] 黄幼田[1] 江亚南[1] 赵明耀[1] 郑智敏[1] 董子明[1] 

机构地区：[1]郑州大学基础医学院病理生理学教研室,河南郑州450052

出　　处：《第四军医大学学报》2009年第17期1568-1570,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30471952);河南省教育厅自然科学研究计划项目(2007310020)

摘　　要：目的:研究互隔交链孢酚(AOH)对食管癌EC9706细胞株中DNA聚合酶β(DNApolβ)表达的影响.方法:以PKA激活剂Forskolin(40μmol/L)为阳性对照,采用免疫细胞化学,WesternBlot方法检测20μmol/LAOH作用EC9706细胞16h后polβ蛋白水平的表达.以PKA特异性抑制剂H89(10μmol/L)预处理EC9706细胞1h,再以20μmol/LAOH作用细胞16h,观察H89阻断AOH激活polβ蛋白表达的作用.结果:免疫细胞化学结果显示,与溶剂对照组和H89处理组相比较,AOH处理组,Forskolin处理组的细胞中polβ表达增高.WesternBlot结果表明,AOH处理组,Forskolin处理组polβ表达分别是溶剂对照组的2.05和3.12倍(P<0.05),而H89组仅为对照组的1.32倍.结论:AOH可以激活食管癌EC9706细胞中polβ基因表达,这可能是通过PKA信号转导通路介导的.AIM： To investigate the expression of DNA polymerase β（ pol β） induced by Altemariol （AOH） in human esophageal squamous cell carcinoma （ESCC） EC9706 cell line and to study the relation between the expression of DNA pol β and PKA signal pathway. METHODS： EC9706 cells were treated with 20 μmol/L AOH and 40 /μmol/L Forskolin （PKA activator） for 16 h. The cells were pretreated with an inhibitor of PKA （H89） for 1 h and then were exposed to 20μmol/L AOH for 16 h. The changes of pol β expression were detected by immunocytochemistry analysis and Western blotting. RESULTS： The results of immunocytochemistry indicated that the expression of pol β increased in the AOH group and Forskolin group, compared with that in control group and H89 group. Western blotting showed that the expression of pol 13 in AOH group and Forskolin group was respectively 2.05 times and 3. 12 times higher than that in control group （ P 〈 0.05 ）, while the expression in the H89 group was only 1.32 times higher than that in control group. CONCLUSION： AOH up-regulates DNA pol β expression in EC9706 cells, which may be mediated by the PKA signal transduction pathway.

关 键 词：DNA聚合酶Β 互隔交链孢酚 蛋白激酶A 信号转导通路 

分 类 号：R735.1[医药卫生—肿瘤]