fas/apo-1、bcl-2蛋白在急性髓系白血病细胞的表达特点及意义  

Protein Expression of bcl-2 and fas/apo-1 and Its Significance in Acute Myeloid Luekemia

作　　者：胡丽华[1] 周剑峰[2] 李崇渔[2] 陈燕[2] 王辨明[2] 

机构地区：[1]同济医科大学附属协和医院输血科,武汉430022 [2]同济医科大学血液病研究所,武汉430022

出　　处：《同济医科大学学报》1998年第2期131-134,共4页Acta Universitatis Medicinae Tongji

基　　金：国家教委留学回国人员启动基金资助课题[教外司留(1995)135号]

摘　　要：用免疫印迹（Western blot）、碱性磷酸酶抗碱性磷酸酶法（APAAP）分析19例急性髓系白血病（AML）骨髓细胞fas/apo-1、bcl-2蛋白表达水平的变化。结果表明绝大部分AML骨髓过表达bcl-2蛋白,部分病例表达fas/apo-1,两者表达呈一定的负相关性。分析结果显示bcl-2蛋白过表达所致的凋亡抑制状态是化疗耐药的主要机制之一,而fas/apo-1的表达是预后较好的特征。The expression of bcl-2 and fas/apo-1 protein in 19 cases of acute meyloid leukemia (AML) was investigated by immuno-alkaline phosphatase (APAAP) and western blot techniques. Great amount of bcl-2 was found in most of the AML cases while comparatively great amount of fas/apo-1 in the other cases. bcl-2 and fas protein expression exhibited a reverse correlation. The results indicated that high level of bcl-2 led to an abnormal apoptosis-induced state which laid a biologic basis for resistance to chemotherapy drugs, while the fas/apo-1 protein expression possibly predicted good response to chemotherapy.

关 键 词：细胞凋亡 FAS/APO-1 BCL-2 白血病 AML 

分 类 号：R733.710.2[医药卫生—肿瘤]