IL-2/HBV PreS融合基因的克隆及序列测定  

Cloning and sequencing of IL 2/HBV preS fusion gene

作　　者：杨晓峰[1] 马文煜[1] 姜绍谆[1] 逯好英[2] 

机构地区：[1]第四军医大学微生物学教研室 [2]军事医学科学院分子遗传研究室

出　　处：《细胞与分子免疫学杂志》1998年第1期29-32,共4页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的：获得ＩＬ２／ＨＢＶｐｒｅＳ融合基因克隆，为表达ＩＬ２／ＨＢＶｐｒｅＳ融合蛋白奠定基础。方法：用ＰＣＲ方法从乙肝全序列中扩增ＨＢＶｐｒｅＳ基因片段，并克隆入带有ＩＬ２基因的ｐｌｙ５质粒中，挑出的阳性克隆再亚克隆到ｐＵＣ１８中进行序列测定。结果：基因全长９３０ｂｐ，编码３１０个氨基酸，序列内有起始码和终止码。结论：所克隆的基因为ＩＬ２与ＨＢＶｐｒｅＳ的融合基因。Aim: To clone the IL 2/HBV preS fusion gene, for expression of IL 2/HBV preS fusion protein. Methods: With PCR technique, the whole gene of HBV preS was amplified and cloned into IL 2 ply5 plasmid vector. The IL 2/HBV preS fusion gene was characterized by nucleotide sequence analysis. Results: The amplification reaction yielded a HBV preS fragment of 525 base pairs. The fusion gene was 930 base pairs in whole length in which the initial codon and terminator were found and 310 amino acids can be encoded. Conclusion: The cloned gene is IL 2/HBV preS fusion gere.

关 键 词：乙型肝炎病毒 白细胞介素2 融合基因 克隆 测序 

分 类 号：Q78[生物学—分子生物学]