人类急性髓系白血病逆转录病毒感染的研究  

Investigation of retroviral infection in human acute myeloid leukemia

作　　者：徐荣臻[1,2] 高其康[1,2] 王世 赵小英[1,2] 徐潍生 李翠琴 王炜琴[1,2] 张克奇 

机构地区：[1]浙江医科大学附属第二医院血液科 [2]浙江农业大学中心实验室

出　　处：《中华医学杂志》1998年第7期504-507,共4页National Medical Journal of China

基　　金：国家自然科学基金

摘　　要：目的探索人类急性髓系白血病（ＡＭＬ）逆转录病毒病因学的可能性。方法应用细胞培养，Ｄ－ＩＧＳＳ，逆转录酶测定，ＸＣ细胞合胞体试验以及电镜技术对白血病细胞和正常造血细胞进行逆转录病毒相关抗原（ＲＶＡＡｇ），逆转录酶活性，病毒致细胞病变效应（ＣＰＥ）及病毒颗粒进行检测与分析。结果３２例ＡＭＬ患者培养前后白血病细胞样本ＲＶＡＡｇ阳性检出率分别为５０．０％和８７．５％。其中１４例ＲＶＡＡｇ阳性样本同时表达逆转录酶活性且ＸＣ细胞合胞体试验阳性。电镜观察证实，７５％ＡＭＬ细胞样本中可见典型的Ｃ型逆转录病毒颗粒。２０例正常对照细胞培养前后样本的ＲＶＡＡｇ，逆转录酶活性，合胞体试验及电镜观察结果均为阴性。结论ＡＭＬ患者的白血病细胞中含有完整的逆转录病毒基因组。Objective To explore the potentiality of retroviral etiology in human acute myeloid leukemia(AML). Methods Cell culture techniques, direct immunogold silver staining(D IGSS), reverse transcriptase(RT)assay, XC syncytium assay,and electron microscopy were employed to evaluate retrovirus associated antigens(RVAAgs), RT activities, CPE, and virus particles of both leukemic cells and normal hematopoietic cells. Results RVAAgs were detected by D IGSS in 50 0% of fresh AML cell samples and 87.5% of cultured AML samples. RT assay showed that RVAAg positive samples from 14 cases simultaneously expressed RT activities, and XC syncytium assay also revealed positive results. Electron microscopy demonstrated typical type C retrovirus particles at various maturation in 75% of cultured leukemic cell samples. In contrast, neither RVAAgs and RT activity nor syncytium and virus particles were detected in normal controls. Conclusions AML cells harbor the entire genome(s) of retrovirus(es), which could release into extracellular spaces in intact particles via budding from the target cells stimulated in vitro by appropriate RV inducing agents. The exact relationship between these viruses and AML etiology is uncertain and needs further study.

关 键 词：白血病 AML 逆转录病毒感染 

分 类 号：R733.71[医药卫生—肿瘤]