伏马菌素FB2间接竞争ELISA检测方法的建立  

Development of an indirect competitive enzyme-linked immunosorbent assay(ic-ELISA) of fumonisin B2

作　　者：李岩松[1,2] 柳增善[2] 周玉[2] 卢士英[2] 王哲[1] 李小兵[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]吉林大学人兽共患病研究所人兽共患病研究教育部重点实验室,吉林长春130062

出　　处：《中国兽医学报》2009年第9期1204-1207,共4页Chinese Journal of Veterinary Science

基　　金：国家"863"计划资助项目(2007AA10Z426);吉林大学农学部青年基金资助项目(4305050102E8)

摘　　要：用卵清白蛋白与伏马菌素B2的偶联物（OVA—FB2）做包被抗原，标准伏马菌素B2（FB2）做竞争抗原，初步建立了检测玉米粉中伏马菌素B2的间接竞争ELISA方法。优化后的ELISA检测方法．线性范围为7．81～250μg／L，最低检测限为6．09μg／L。曲线回归方程为y=7．038x＋120．25，R^2=0．9835，批内平均变异系数为3．92％，批间平均变异系数为5．53％。对玉米粉加标样品测定结果表明，利用甲醇-EDTA法提取样品的平均加标回收率达到了96．11％。An ic-ELISA for detecting fumonisin B2（FB2） of maizena was developed by using the coating antigen of OVA-FB2 and competitive antigen of FB2. The linear range was 7. 81-250 μg/L and the limited detection was 6.09 μg/L. The regression equation of standard curve was y= -47. 038x＋120.25 （the correlation coefficient R^2 = 0. 983 5）. The variability of intra-assay and inter assay of the standard curve of the ic-ELISA were 3. 92% and 5.53%, respectively. The mean recovery rate was 96.11% of the FB2 in maizena extracted by methanol-EDTA.

关 键 词：玉米粉 伏马菌素B2(FB2) 间接竞争ELISA 

分 类 号：S852.66[农业科学—基础兽医学] S856.9[农业科学—兽医学]