纤维素酶法提取枸骨叶熊果酸的工艺研究  

Studies on the Technology of Extracting Ursolic Acid from Folium Ilicis Cornutae by Cellulase

作　　者：曾超珍[1] 郭玉[1] 孙丽香[1] 

机构地区：[1]中南林业科技大学生命科学与技术学院,湖南长沙410004

出　　处：《北方园艺》2009年第9期216-218,共3页Northern Horticulture

基　　金：中南林业科技大学青年科学研究基金资助项目(06007B);中南林业科技大学林木遗传育种学科资助项目

摘　　要：研究纤维素酶法提取枸骨叶熊果酸最佳工艺条件。以枸骨叶熊果酸提取率为指标,通过单因素和正交试验优化酶法提取枸骨叶熊果酸的工艺。结果表明:纤维素酶酶解工艺的最佳条件为pH 5.2,酶量6 mg/g,酶解温度50℃,酶解时间3 h。验证试验结果表明该提取工艺稳定可行,适用于枸骨叶熊果酸的提取。To optimize the extraction technology for ursolic acid of Folium Ilicis Cornutae by cellulase.The extraction technology for ursolic acid of Folium Ilicis Cornutae was optimized by single factor and orthogonal experiment with the use of the extraction rate of ursolic acid as the assessment index.The optimal enzymolysis conditions were as follows： the pH of extracting media was 5.2,the amounts of cellulose was 6 mg/g,the enzymolysis temperature was 50℃and the enzymolysis time was 3 h.It was shown by an approval test that the optimized technology was stable,feasible and suitable for the extraction of ursolic acid of Folium Ilicis Cornutae.

关 键 词：纤维素酶 枸骨叶 熊果酸 正交试验 

分 类 号：S567.19[农业科学—中草药栽培]