苔藓植物RAPD应体系的建立及遗传多样性分析  

Establishment of RAPD Reaction System and Analysis of Genetic Diversity for Bryophytes

作　　者：张安世[1] 刘永英[1] 张为民[1] 邢智峰[1] 辛泽华[1] 

机构地区：[1]焦作师范高等专科学校,河南焦作454001

出　　处：《河南师范大学学报（自然科学版）》2009年第5期122-125,共4页Journal of Henan Normal University(Natural Science Edition)

基　　金：河南省科技攻关项目(0624240019);河南省教育厅自然科学研究计划项目(2008C180001;2008C180002);河南省高校青年骨干教师资助计划项目

摘　　要：以多枝青藓为材料优化RAPD反应条件,在此基础上对11种苔藓植物进行遗传多样性分析.结果表明:苔藓植物RAPD反应(25μl体系)的最佳条件为,Taq酶,1.0U;Mg2+浓度,2.0 mmol/L;dNTP浓度,0.2 mmol/L;模板DNA,60 ng;引物浓度,10 pmol.用40条引物进行扩增筛选,有6条引物扩增条带清晰,重复性好,共扩增出77条带.通过SPSS11.5分析软件对扩增结果进行聚类分析,结果与形态学分类基本一致,说明RAPD技术可用于苔藓植物的遗传多样性研究.An optimal protocol of Random Amplified Polymorphism DNA （RAPD） is established for Brachythecium fasciculirameum. Based on it, analyses of genetic diversity are conducted with eleven species Of bryophytes. The results show that the optimal protocol is accomplished in 25 μL reaction volumes containing 1 unit Tag DNA polymerase, 2.0 mmol/L MgCl2 ,0. 2 mmol/L dNTPs, 60 ng template DNA and 10 pmol/L RAPD primer. Six primers are selected from forty ones to generate seventy-seven DNA bands. A dendrogram is established based on Hierarchical cluster analysis by 11.5 SPSS, which is similar to morphological studies. It is clear that the RAPD technology can be used to analyze the genetic diversity of bryophytes.

关 键 词：苔藓 RAPD 亲缘关系 遗传多样性 

分 类 号：Q781[生物学—分子生物学]