磁性纳米Fe_3O_4颗粒逆转卵巢癌SKOV3/DDP耐药性的研究  

A study on the reversing effect of Fe_3O_4-magnetic nanoparticles on DDP resistance in the ovarian carcinoma cell(SKOV3/DDP) strain

作　　者：姜智[1,5] 陈宝安[1] 吴强[3] 夏国华[1] 张宇[1] 周颐[1] 徐翠荣[1] 程坚[1] 高峰[1] 李国宏[1] 陈文姬[1] 刘莉洁 李小毛[4] 王雪梅[1] 

机构地区：[1]东南大学临床医学院附属中大医院,南京210009 [2]生理教研室 [3]江苏省肿瘤医院妇产科 [4]D-66041德国萨尔布吕肯萨尔大学医学部 [5]江苏省肿瘤医院

出　　处：《江苏医药》2009年第9期1045-1047,共3页Jiangsu Medical Journal

基　　金：国家自然科学基金(39970832;30740062)

摘　　要：目的探讨磁性纳米四氧化三铁颗粒(Fe3O4-MNPs)逆转人卵巢癌顺铂耐药细胞株(SKOV3/DDP)耐药性的可能性。方法将SKOV3/DDP细胞分为对照组、顺铂(DDP)组、Fe3O4-MNPs组和DDP+Fe3O4-MNPs组,体外培养后MTT法测定细胞生长半数抑制浓度(IC50),流式细胞术测定细胞凋亡及电感耦合等离子体原子发射光谱(ICP)法测定细胞内DDP含量。结果DDP组IC50为39.31μM,DDP+Fe3O4-MNPs组为17.4μM(P<0.05)。DDP+Fe3O4-MNPs组细胞凋亡率较DDP组明显提高(P<0.05)。DDP+Fe3O4-MNPs组细胞内顺铂含量明显高于DDP组(P<0.05)。结论Fe3O4-MNPs可与化疗药物顺铂协同,逆转卵巢癌细胞对DDP的耐受性。其作用可能是通过提高细胞内药物聚集量实现的。Objective To study the possibility of Fe3O4-MNPs reversing cisplatin （DDP） resistance to ovarian carcinoma cell （SKOV3/DDP）. Methods The SKOV3/DDP strains were divided into DDP treatment （group A）, Fe3O4 magnetic nanoparticles treatment （group B）, DDP ＋ Fe3 O4- MNPs treatment（group C）, and control group（E）. The proliferation inhibition （median inhibitory concentration, IC50 ） was measured by MTT, the apoptosis by flow cytometry, and the intracellular DDP level by inductively-coupled plasma atomic emission spectrometry （ICP）. Results The ICs0 in group C was 17.4 μM, which was significantly less than 39.31 μM in group A （P〈0.05）. The apoptosis rate of SKOV3/DDP cells was higher in group C than that in group A （P〈0.05）. The intraeellular cisplatin level in group C was higher than that in group A （P〈0. 05）. Conclusion Fe3 O4-MNPs can reverse the DDP resistance to the ovarian carcinoma cell （SKOV3/DDP）, which may be related to its effect of increasing the intracellular accumulation of DDP.

关 键 词：卵巢癌 磁性纳米四氧化三铁颗粒 SKOV3/DDP耐药 

分 类 号：R737[医药卫生—肿瘤]