苦瓜降糖多肽PA对四氧嘧啶糖尿病小鼠的口服降血糖作用  

Hypoglycemic effect of oral PA extracted from momordica Charantia Seeds on alloxan-induced diabetic mice

作　　者：刘红雨[1] 付中平[1] 周吉燕[1] 王富军[1] 胡之璧[1] 

机构地区：[1]上海中医药大学中药研究所,上海201203

出　　处：《中药药理与临床》2009年第4期32-35,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家"十一五"科技支撑计划项目(2006BAI06A16)

摘　　要：目的:研究添加蛋白水解酶抑制剂PI的苦瓜降糖多肽PA口服降血糖作用及其可能的作用机制。方法:小鼠尾静脉注射四氧嘧啶溶液80mg/kg造糖尿病小鼠模型,分别进行PA皮下注射降血糖实验和PA口服降血糖实验;21日实验结束时取材测定血糖、血清胰岛素水平、小肠粘膜α-葡萄糖苷酶活性、骨骼肌己糖激酶(HK)活性,固定胰腺进行病理观察。结果:皮下注射PA显著降低小鼠血糖值。口服PA组小鼠血糖值明显减低;血清胰岛素水平明显升高;小肠粘膜催化麦芽糖生成的葡萄糖量大幅降低;骨骼肌己糖激酶活性明显增强;四氧嘧啶对小鼠胰岛的选择性损伤得到缓解。结论:初步证明了添加酶抑制剂PI的苦瓜降糖多肽PA口服降血糖作用;其作用机制可能是抑制小肠粘膜α-葡萄糖苷酶活性;增强骨骼肌己糖激酶活性;保护或修复胰岛β细胞,促进或改善胰岛素分泌。Objective：To investigate the hypoglycemic effect of oral PA and its possible mechanisms.Methods：PA,co-administration with proteinase inhibitors（PI） also from Momordica charantia seeds,was orally administrated into alloxan-induced diabetic mice（100mg/kg,PI 50mg/kg）.Blood glucose level,insulin level,hexokinase activity in skeletal muscle and α-glucosidase activity of intestinal mucosa were measured.The pancreas was fixed with 10% formaldehyde for histopathological examination.Results：Daily treatment with PA（co-administration with PI ） for 3 weeks led to fall in blood glucose level at 14 days and at 21 days, respectively. In addition, PA also raised serum insulin concentra- tions. The increased c^-glucosidase activity of intestinal mucosa and decreased hexokinase activity in skeletal muscle were also restored partly. Oral PA may facilitate tile recovery of partially damaged islet cells in alloxan-induced diabetic mice. Conclusion：The late hypoglycemie effect of oral PA, is probably due to its insulin secretagogue effect, reparative action on damaged islet ceils and restoration of glucose metabolism balance.

关 键 词：苦瓜降糖多肽PA 酶抑制剂PI 胰岛素 Α-葡萄糖苷酶 己糖激酶 

分 类 号：R285[医药卫生—中药学]