瘦素在肝纤维化组织中的表达及其与肝星状细胞活化的关系  

Expression of leptin in hepatic fibrosis and its relation with hepatic stellate cell activation

作　　者：许晶[1] 朱净[1] 潘亮[1] 陆静贤[1] 肖明兵[1] 黄华[2] 倪润洲[1] 陆翠华[1] 

机构地区：[1]南通大学附属医院消化科,江苏省南通市226001 [2]南通大学附属医院病理科,江苏省南通市226001

出　　处：《世界华人消化杂志》2009年第21期2127-2130,共4页World Chinese Journal of Digestology

基　　金：南通市科技局社会发展基金资助项目;No.2007030~~

摘　　要：目的:探讨瘦素(leptin)在肝纤维化组织中的表达及其与肝纤维化过程中转化生长因子(TGF-β1)以及α平滑肌肌动蛋白(α-SMA)表达的相关性,了解leptin与肝星状细胞(HSC)活化的关系.方法:健康♂SD大鼠40只,随机分成正常对照组和CCl4诱导的肝纤维化模型组.于造模2、4、6wk末分批处死动物,分别采用RT-PCR和Westernblot、免疫组织化学方法联合检测leptin、TGF-β1及α-SMA在肝纤维化组织中的表达.结果:正常对照组肝脏组织中leptin、TGF-β1、α-SMA均有微量表达;CCl4注射2wk后,leptin、TGF-β1、α-SMA表达开始增强,2、4、6wk肝组织中的表达强度呈明显递增趋势(P<0.05).leptin与TGF-β1和α-SMA表达均呈显著相关性(r=0.668,0.570,均P<0.05).结论:leptin的阳性表达随着肝纤维化程度的加重而增强.在肝纤维化过程中leptin可能参与了HSC活化、增殖以及ECM的合成.AIM：To investigate the expression of leptin in hepatic fibrosis and explore its correlation with the expression of TGF-β1 andα-SMA as well as its relation with the activation of hepatic stellate cells（HSC）. METHODS：Forty healthy male SD rats were randomly divided into normal control group and carbon tetrachloride treatment group.The rats were given a subcutaneous injection of either vehicle or carbon tetrachloride,and sacrificed at weeks 2,4 and 6 after the injection,respectively.The mRNA and protein expression of leptin, TGF-β1 and α-SMA was assayed t）y reverse transcription-polymerase chain reaction （RT-PCR）, Western blot and immunohistochemistry, respectively.RESULTS： Leptin, TGF-β1 and α-SMA were lowly expressed in normal liver tissues. After injection of carbon tetrachloride, the expression of leptin, TGF-β1 and α-SMA began to increase at week 2 and was significantly higher at weeks 4 and 6 （P 〈 0.05）, showing a gradually rising trend. The expression of leptin was positively correlated with that of TGF-β1 and α-SMA （r = 0.668 and 0.570, respectively; both P 〈 0.05）. CONCLUSION： The expression of leptin, TGF-β1 and α-SMA increases with the development of hepatic fibrosis. Leptin is probably involved in the activation of HSCs and the synthesis of extracellular matrix （ECM） in the development of hepatic fibrosis.

关 键 词：肝纤维化 瘦素 转化生长因子 肝星状细胞 Α平滑肌肌动蛋白 

分 类 号：R575.2[医药卫生—消化系统]