NFkB1基因启动子区域多态性与中国湖北汉族人溃疡性结肠炎的关系  

Association of NFkB1 -94ins/delATTG promoter polymorphism with ulcerative colitis in Chinese Han population of Hubei Province

作　　者：雷媛[1] 邓长生[1] 

机构地区：[1]武汉大学中南医院消化内科,湖北省武汉市430071

出　　处：《世界华人消化杂志》2009年第21期2212-2216,共5页World Chinese Journal of Digestology

摘　　要：目的:研究NFkB1基因启动子-94ins/del ATTG与中国汉族人溃疡性结肠炎的相关性.方法:采用PCR限制性片段长度多态性分析(PCR-RFLP)法,检测73例中国湖北汉族溃疡性结肠炎患者与121例健康对照者NFkB1基因启动子-94ins/del ATTG基因型,分析该基因多态性与溃疡性结肠炎以及临床亚型的相关性,并将该结果与已报道的意大利人群中该位点多态性分布进行比较.结果:在NFkB1基因启动子-94ins/del ATTG位点UC组的基因型频率、等位基因频率、临床亚型与正常对照组比较无显著相关性(P>0.05);然而,中国湖北汉族人群与意大利高加索人群在该位点的基因型(χ2=13.155,P<0.05)和等位基因(OR=0.566,95%CI0.413-0.774,P<0.01)频率分布有显著差异.结论:NFkB1基因启动子区域多态性与中国湖北汉族人溃疡性结肠炎无显著相关性.AIM：To investigate the association between NFkB1-94ins/delATTG promoter polymorphism and ulcerative colitis in Chinese Han population of Hubei Province. METHODS：The NFkB1–94ins/delATTG promoter polymorphism was genotyped in 73 unrelated Chinese patients with ulcerative colitis and 121 healthy controls using polymerase chain reaction-based restriction fragment length polymorphism（PCR-RFLP）.The correlation of allele and genotype frequencies for this locus with the clinical subtypes of ulcerative colitis was analyzed.The results were compared with those obtained in a previous study of Italian population.RESULTS： No significant differences were found in genotype and allele frequencies of the NFκB1-94ins/delATTG promoter polymorphism between patients with ulcerative colitis and healthy controls. There was no significant correlation between allele and genotype frequencies for this locus and the clinical subtypes of ulcerative colitis （P 〉 0.05）. However, the genotypes （Χ^2 = 13.155, P 〈 0.05） and allele frequencies （OR = 0.566, 95%CI： 0.413-0.774, P 〈 0.01） for this locus were significantly different between the Chinese and Italian populations.CONCLUSION： No association between NFκB1 -94ins/delATTG promoter polymorphism and ulcerative colitis is found in Chinese Han popu- lation of Hubei Province.

关 键 词：溃疡性结肠炎 NFkB1 基因多态性 

分 类 号：R574.62[医药卫生—消化系统]