缬沙坦对血管平滑肌细胞迁移及丝裂原活化蛋白激酶的影响  

Valsartan on migration of vascular smooth muscle cell via MAPK pathway

作　　者：徐杰丰[1] 严松彪[1] 陈晖[1] 高红丽[1] 南芳[1] 王永亮[1] 李虹伟[1] 

机构地区：[1]首都医科大学附属北京友谊医院心血管中心,北京市100050

出　　处：《中华老年多器官疾病杂志》2009年第4期364-367,369,共5页Chinese Journal of Multiple Organ Diseases in the Elderly

基　　金：教育部博士点基金(20050025012)

摘　　要：目的观察缬沙坦(Val)对血管紧张素Ⅱ(AngⅡ)刺激下大鼠血管平滑肌细胞(VSMC)迁移及磷酸化42/44丝裂原活化蛋白激酶(p42/44MAPK)表达的影响。方法组织贴块法培养大鼠胸主动脉平滑肌细胞,tran-swell小室检测细胞的迁移能力,免疫印迹法检测p42/44 MAPK蛋白表达的水平。结果(1)AngⅡ能明显促进VSMC迁移,该作用可被Val和MAPK激酶的特异性抑制剂PD98059所抑制。(2)AngⅡ刺激VSMC 5min时,p42/44 MAPK的表达量最大,该作用可被Val和PD98059所抑制。(3)Val单独作用于VSMC时,对细胞的迁移及p42/44 MAPK的表达均无明显影响。结论Val抑制AngⅡ诱导的VSMC迁移与其抑制AngⅡ诱导的p42/44MAPK的表达相关。Objective To determine the effect of valsartan（val） on the migration of isolated rat vascular smooth muscle cell（VSMC） and the expression of phospho-42/44 mitogen-activated protein kinase（p42/44 MAPK） promoted by angiotensin Ⅱ. Methods VSMC from the rat thoracic aorta was cultured by attachment-block culture. VSMC migration was measured by migration chamber system, p42/44 MAPK expression was determined hy Western blot. Results （1） Angiotensin Ⅱ obviously promoted VSMC migration, the effect was inhibited by valsartan and PD98059. （2）Angiotensin Ⅱ obviously promoted p42/44 MAPK expression,and the expression was at maximal in 5 min,the effect was inhibited by val and PD98059. （3）Compared with the control, val didn＇t inhibit VSMC migration and p42/44 MAPK expression. Conclusion The effect that val inhibits angiotensin Ⅱ-induced VSMC migration is related to its inbibiting angiotensin Ⅱ-induced p42/44 MAPK expression.

关 键 词：肌 平滑 血管 缬沙坦 血管紧张素Ⅱ 丝裂原活化蛋白激酶 

分 类 号：R361.3[医药卫生—病理学] R644[医药卫生—基础医学]