细胞因子的相互作用对角膜基质细胞胶原代谢的影响  

Effect of interaction of cytokine on collagen degradation by keratocytes

作　　者：姜耀和 谷树严[2] 郝继龙[2] 王晓东[2] 

机构地区：[1]公主岭市第三人民医院眼科,吉林公主岭136105 [2]吉林大学中日联谊医院眼科,吉林长春130033

出　　处：《中国实验诊断学》2009年第8期1005-1006,共2页Chinese Journal of Laboratory Diagnosis

摘　　要：目的观察细胞因子IL-1α与TGF-β对兔角膜基质细胞胶原降解的作用,并探讨其相互作用对角膜基质细胞胶原分解代谢的影响。方法角膜基质细胞表面添加含纤溶酶原及不同浓度的IL-1α和/或TGF-β的MEM液,培养24小时,测定培养液中的羟脯氨酸含量作为胶原降解活性。结果IL-1α促进角膜基质细胞胶原降解;TGF-β1抑制角膜基质细胞的胶原降解,IL-1α与TGF-β同时存在,在IL-1α低浓度时,抑制角膜基质细胞胶原降解,高浓度时,TGF-β1不能抑制角膜基质细胞胶原降解。结论IL-1α与TGF-β的相互作用调控角膜基质细胞胶原代谢,在角膜基质创伤修复过程中起着重要作用。Objective To observed effect of IL-1α and TGF-β on collagen degradation and its interaction on collagen matablism of keratocytes.Methods The activity of collagen degradation of three dimentional culture of keratocytes in collagen gel was determined with spectrophotometer.Results IL-1α increased collagen degradation of keratocytes;TGF-β,suppressed collagen degradation of keratocytes.In the presence both IL-1α and TGF-β,suppressed collagen degradation in low concentration of IL-1α;could not suppressed collagen degradation in high concentration of IL-1α. Conclusion Interaction of cytokines could regulated collagen matablism of keratocytes, which probably play a important role in the wound healing of cornea stroma.

关 键 词：细胞因子 胶原降解 角膜基质细胞 

分 类 号：R772.2[医药卫生—眼科]