姜黄素衍生物FM17对K562细胞增殖影响及其与P210^bcr/ab1激活的信号途径关系  

The effect of curcumine derivative FM17 on the proliferation of K562 cells and its association with P210^bcr/ab1 initiated signal pathway

作　　者：陈云艳[1] 许建华[1] 

机构地区：[1]福建医科大学药学院,福建医科大学临床药理所,福建福州350004

出　　处：《公共卫生与临床医学》2009年第2期90-93,共4页Public health and dinical medicine

基　　金：国家自然科学基金资助项目（编号：30873101）

摘　　要：目的探讨姜黄素衍生物FM17对体外培养的慢性粒细胞白血病（CML）急变期细胞K562细胞增生的影响，及其-9p210^bcr/ab1激活的信号途径的关系。方法四甲基偶氮唑蓝（MTT）法检测姜黄素衍生物不同时间点和不同的浓度对K562细胞增殖的抑制作用。Western blot的方法分析姜黄素衍生物FM17不同浓度、不同时间点对P210b刊曲。激活的信号的影响。结果2-8μg／mL浓度的FM17在12h、24h、36h及48h均可明显抑制K562细胞增殖，并在一定范围内呈时间和剂量依赖性；FM17可抑制P210^bcr/ab1蛋白的含量，且可下调P210^bcr/ab1激活的下游信号分子PKC和p—Erk。结论姜黄素衍生物FM17较姜黄素有更强的抗增殖能力，对P210^bcr/ab1及其激活的信号途径也有较强的抑制作用。Objective To investigate the effect of curcumine derivative FM17 on the proliferation of ^Bcr/ab1（＋） K562 cell line and P210^bcr/ab1 initiated signal pathway. Methods MTT assay was used to determine the inhibition of the proliferation of K562 cell at different time-points with different dosages. The effect on P210^bcr/abl2 initiated signal pathway was determined by western blotting. Results The exposure of K562 cells to 2-8μg/ml curcumine ramification could reduce the proliferation of K562 cells with a dose- and time-dependent pattern. In addition, the curcumine ramification could directly inhibit the expression of p210 protein and down-regulate the expression of downstream protein such as PKC, p-Erk, HSP70. Conclusions Curcumine ramification has a stronger inhibitory effect on the proliferation of K562 than curcumine itself, and it could suppress the p210 initiated signal pathway by down-regulating multiple protein expression.

关 键 词：姜黄素衍生物 K562 P210^bcr/ab1 p-stat5 P-ERK 

分 类 号：R73-34[医药卫生—肿瘤]