PD、SB对表皮生长因子诱导的大鼠VSMCS增殖及迁移的影响  

Effects of PD and SB on EGF-induced proliferation and migration in vascular smooth muscle cells

作　　者：陈龙菊[1] 张复贵[2] 唐杰[1] 姜铧[3] 孙晓东[4] 丁妍[5] 张兴华[1] 王汉琴[1] 

机构地区：[1]郧阳医学院解剖学教研室,十堰442000 [2]郧阳医学院附属东风公司总医院 [3]郧阳医学院附属太和医院血液科 [4]郧阳医学院基础医学研究所 [5]郧阳医学院附属太和医院生命科学研究所

出　　处：《陕西医学杂志》2009年第9期1115-1117,共3页Shaanxi Medical Journal

基　　金：郧阳医学院博士启动金资助(2007QDJ7)

摘　　要：目的:探讨ERK1/2抑制剂PD98059、p38MAPK抑制剂SB202190对表皮生长因子(EGF)诱导的血管平滑肌细胞(VSMCs)增殖及迁移的影响。方法:实验分为对照组、EGF组、PD98059(PD)组、SB202190(SB)组。以低血清培养的VSMCs作为对照,采用细胞计数、划痕损伤实验,观察PD98059和SB202190对EGF诱导的VSMCs增殖及迁移的影响。结果:干预后24h,EGF组VSMCs增殖、迁移较对照组明显增强,加用PD98059和SB202190后,明显抑制了VSMCs的增殖和迁移。结论:EGF诱导的VSMCs增殖和迁移可能是通过p38MAPK和ERK1/2信号通路发挥作用。Objective： To explore the effects of PD98059, inhibitor of ERK1/2 and SB202190, inhibitor of p38MAPK on proliferation and migration of EGF -induced vascular smooth muscle cells （VSMCs）. Methods： There were four groups in this experiment, including control, EGF group, PD98059 （PD） group and SB202190（PD） group. Using rat VSMCs exposed to low serum culture medium as control. The proliferation of the VSMCs was measured by cell counting. The migration rate of the VSMCs was observed by wound healing assay. Results： After 24h stimulation , EGF induced VSMCs proliferation and migration, whereas PD98059 and SB202190 inhibited significantly these role, moreover, the inhibiting role of the latter was more obvious. Conclusion： These results suggest that EGF-induced VSMCs proliferation and migration may play a role through ERK1/2 and p38MAPK signal pathways.

关 键 词：肌 平滑 血管/病理生理学 表皮生长因子 模型 动物 大鼠 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R394.33[医药卫生—临床医学]