斑鳠血清促性腺激素的季节变化和埋植甲基睾酮对斑鳠促性腺激素分泌的影响  

Seasonal Changes of Serum Gonadotropin Levels and Effects of MT Implantation on GtH Secretion of Spotted Longbarbel Catfish(Mystus guttatus)

作　　者：周立斌[1,2] 刘晓春[1] 马细兰[2] 林浩然[1] 李大疆[3] 陈焜慈[3] 李恒颂[3] 邬国民[3] 

机构地区：[1]中山大学有害生物控制与资源利用国家重点实验室//水生经济动物研究所和广东省水生经济动物良种繁育重点实验室,广东广州510275 [2]惠州学院生命科学系和生物技术研究所,广东惠州516007 [3]中国水产科学研究院珠江水产研究所,广东广州510380

出　　处：《中山大学学报（自然科学版）》2009年第5期103-107,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省自然科学基金资助项目(06027456);农业部热带亚热带鱼类选育与养殖重点开放实验室开放基金资助项目

摘　　要：采用鲤鱼GtHβ-亚基(cGtHβ)的标准品和抗血清建立了斑鳠异源的促性腺激素(Gonadotropin,GtH)放射免疫测定技术,并对斑鳠性腺指数(Gonadosomatic index,GSI)和GtH分泌的季节变化,以及埋植甲基睾酮(17α-methyl-testosterone,MT)对斑鳠GtH分泌的影响进行了研究。结果表明:斑鳠雌雄鱼GSI值分别在5月份和6月份达到最高,与其他月份相比有显著性提高(P<0.05);斑鳠雌鱼血清GtH水平在6月份达到最高,与10月和12月相比有显著性提高(P<0.05),斑鳠雄鱼血清GtH水平在6月份达到最高,4月、10月和12月相比有显著性提高(P<0.05);雌雄鱼血清GtH水平表现出基本相同的变化趋势,并且血清GtH水平差异不显著(P>0.05);埋植MT对斑鳠雌雄鱼血清GtH水平有一定影响,在性腺发育初期能提高雌雄鱼血清GtH水平,而在性腺成熟和排卵期能降低雌雄鱼血清GtH水平。上述结果表明在斑鳠的人工繁殖中可以采用埋植MT的方法对斑鳠进行催熟和催产。The seasonal changes of GSI and serum gonadotropin （GtH） levels of spotted longbarbel cat-fish （Mystus guttatus Lacepede）, and effect of αor-methyl-testosterone （MT） implantation on GtH secretion were studied. Based on carp gonadotropin-β （cGtH-β） and its anti-serum （Rac）, heterologous radio-immunoassay （RIA） for GtH of spotted longbarbel catfish was developed. These results showed go- nadosomatic index （GSI） of female and male spotted longbarbel had the highest value in May and June, and was higher than those in other monthssignificantly （P 〈 0. 05 ） ; Serum GtH levels of female andmale spotted longbarbel catfish had the （female in Oct and Dec, male in Apr, highest value in June, and was higher than those in other months Oct and Dec） significantly （ P 〈 0. 05 ） ; The seasonal changes of serum GtH levels of male spotted longbarbel catfish were similar to those of female spotted longbarbel catfish, and no significant differences were observed among female fish with male fish in all months; MT implantation had effect on serum GtH levels of spotted longbarbel catfish, in early gonada development stage gonadal maturation and ovulation stage, MT implantation could increase serum GtH level of spotted longbarbel catfish, but in gonadal maturation and ovulation stage, MT implantation could decrease serum GtH level of spotted longbarbel catfish. These results indicated that MT implantation could have an effect on artificial propagation of spotted longbarbel catfish.

关 键 词：斑鳠 促性腺激素 季节变化 MT 

分 类 号：Q579.1[生物学—生物化学]