一氧化氮对鼻咽癌细胞生长及血管内皮生长因子的调节作用  

Effect of nitric oxide on growth of nasopharyngeal carcinoma cell lines and expression of vascular endothelial growth factor

作　　者：易翔[1] 唐安洲[1] 覃颖[2] 温文胜[1] 谢莹[3] 

机构地区：[1]广西医科大学第一附属医院 [2]广西壮族自治区人民医院耳鼻咽喉科 [3]西医科大学实验中心

出　　处：《中国癌症防治杂志》2009年第3期197-199,202,共4页CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT

基　　金：广西壮族自治区科技攻关资助项目(桂科攻0322024-1A)

摘　　要：目的通过体外实验检测一氧化氮(nitric oxide,NO)对鼻咽癌细胞株生长的影响及对血管内皮生长因子(vascular endothelial growth factor,VEGF))表达的调节作用。方法以外源性NO供体S-亚硝基-N-乙酰基-DL-青霉胺(S-nitroso-N-acetylpenicillamine,SNAP)对鼻咽癌细胞系TWO3及CNE2进行处理,采用硝酸还原酶法测定细胞培养液中NO浓度,并以MTT法检测NO对鼻咽癌细胞系存活率的影响;逆转录聚合酶链反应(RT-PCR)及免疫印迹法(westernblot)法检测NO对鼻咽癌细胞系的VEGF调节作用。结果低浓度的NO对鼻咽癌细胞的生长无明显抑制作用,高浓度NO能抑制TWO3及CNE2生长,且NO能调节鼻咽癌细胞株的VEGF的表达。结论一定程度增高的NO浓度可以在鼻咽癌细胞中起到细胞信号传导作用,调节VEGF的表达,参与鼻咽癌的发生发展过程;而过高浓度的NO则可能抑制鼻咽癌的发展。Objections To study the effective of nitric oxide （NO） on growth of nasopharyngeal carcinoma （NPC） cell lines, and on modulation of VEGF expression. Methods NPC cell lines CNE2 and TWO3 were treated with NO donor S-nitroso-N- acetylpenicillamine （SNAP）. NO concentrations in cell culture medium were detected by nitrate reductase method. The survival rates of NPC cell lines were detected by MTT method. The expression of VEGF was detected by reverse transcription （RT-PCR） and western blot. Results NO inhibited growth of NPC cell lines in higher concentrations, while the low concentration of NO had no significant effect on NPC cell lines survival rate. NO modulated the expression of VEGF in NPC cell lines. Conclusions NO as a signal molecule plays a role in modulating the expression of VEGF, therefore it contributes to the development of NPC. Meanwhile, the high concentrations of NO can inhibit the development of NPC.

关 键 词：鼻咽癌 一氧化氮 血管内皮生长因子 细胞系 

分 类 号：R739.63[医药卫生—肿瘤]