B细胞非霍奇金淋巴瘤TCR基因重排及CDR3谱型分析  

Analyses of the TCR β-chain gene rearrangement and CDR3 repertoire in patients with B-NHL

作　　者：张建波[1] 宋永平[2] 于庆凯[1] 胡俊[3] 董涛[3] 

机构地区：[1]河南省肿瘤医院病理科,郑州450003 [2]河南省肿瘤医院血液科,郑州450003 [3]中山大学中山医学院微生物学教研室

出　　处：《白血病．淋巴瘤》2009年第9期556-558,共3页Journal of Leukemia & Lymphoma

摘　　要：目的分析B细胞淋巴瘤穿刺标本中T细胞受体（TCR）β链克隆性基因重排及互补决定区3（CDR3）谱型。方法应用RT—PCR扩增TCR Vβ24个亚家族的CDR3区基因，并经基因扫描确定T细胞克隆陛，对提示单克隆或寡克隆增生亚家族的PCR产物进行测序。结果3例淋巴瘤患者TCR存在限制性取用，仅表达2～5个Vβ亚家族。所有患者均存在克隆性增生T细胞，增生形式包括寡克隆及寡克隆增生趋势。CDR3区序列分析证实增生的T细胞克隆具有不同的氨基酸序列。结论B细胞淋巴瘤患者存在T细胞克隆性增生，其TCRVB呈限制性取用，不同克隆T细胞的CDR3序列不同。Objective To analyze the clonal gene rearrangement and complementarity determining region 3 （CDR3） Repertoire of TCR G-chain in fine needle aspiration biopsy （FNAB） specimens of B-cell lymphoma. Methods The TCR CDR3 region genes of 24 TCR Vbeta subfamilies were amplified by utilizing RT-PCR technology, and the CDR3 lengths of TCR β-chain were analyzed with GeneScan technology for 4 healthy individuals and 3 patients with B-cell lymphoma. The clonality of T ceils presumed by spectratyping was further confirmed by CDR3 sequencing. Results TCR β-chain presented specific repertoire skewing in 3 cases, and only 2-5 TCR Vbeta subfamily T cells were identified, respectively. Clonal expanded T cells, including oligoclonal, oligoclonal trend patterns, in one or more Vbeta subfamilies were found in all cases. The oligoclonal expanded T cells have different CDR3 amino acid sequences. Conclusion There were characteristic T cells clone proliferation and selected usage of TCR Vbeta subfamily T cells could be found in 3 cases with B-cell lymphoma. The sequences of CDR3 in different TCR clone proliferation are mostly different.

关 键 词：淋巴瘤 B细胞 基因重排 互补决定区 寡核苷酸序列分析 

分 类 号：R733.1[医药卫生—肿瘤]