高效液相色谱法测定水产品中四种氟喹诺酮类药物残留  

HPLC Determination of Four Fluoroquinolones in Aquatic Products

作　　者：张林田[1] 黄少玉 陈小雪[1] 张冬辉[1] 陈建伟[1] 苏建晖[1] 

机构地区：[1]汕头出入境检验检疫局,汕头515041 [2]汕头市疾病预防与控制中心,汕头515031

出　　处：《理化检验（化学分册）》2009年第9期1086-1087,1090,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：提出了反相高效液相色谱法同时测定水产品中氧氟沙星、诺氟沙星、环丙沙星和恩诺沙星药物的残留量。样品经乙腈-乙酸提取,正己烷净化,高效液相色谱分离,荧光检测器检测,外标法定量。以MERK C_(18)色谱柱(4.6 mm×250 mm,5μm)为分离柱,以乙腈和四丁基溴化铵以体积比6比94的混合液为流动相。4种氟喹诺酮类化合物质量浓度与其峰面积呈线性关系。标准加入平均回收率在93.0%～111.3%之间,相对标准偏差(n=8)均小于10%。RP-HPLC was applied to the determinations of fluoroquinolones including ofoxacin, norfloxaein, eiprofloxaein and enrofloxacin, in aquatic products. The sample was extracted by aeetonitrile and acetic acid mixed solution, the extract obtained was purified with n-hexane. The MERK C18 column （4. 6 min×150 mm, 5 μm） was used as stationary phase, and a mixed solution of aeetonitrile and tetrabutyl ammonium bromide （mixed in the a ratio of 6 to 94） was used as mobile phase. The eluate from column was used for HPLC analysis with fluorescence detector. External standard calibration was adopted in the determination. Linear relationships between the values of peak area and mass concentration of 4 fluoroquinolones were obtained. The values of recovery found by standard addition method were in the range of 93.0%-111.3%, and values of RSD＇s （n=8） were less than 10%.

关 键 词：高效液相色谱 水产品 氟喹诺酮类 

分 类 号：O652.63[理学—分析化学]