内皮细胞特异性分子1在原发性肝癌中的表达及其意义  

Expression of ESM-1 in hepatocellular carcinoma is associated with angiogenesis and tumor invasion

作　　者：向昕[1] 赵文博[1] 王欣[1] 

机构地区：[1]山东大学附属省立医院血液科,济南250021

出　　处：《中华肝脏病杂志》2009年第9期661-664,共4页Chinese Journal of Hepatology

基　　金：山东省科技攻关项目（2006GG2202007）

摘　　要：目的探讨内皮细胞特异性分子1（ESM-1）在原发性肝癌中的表达情况及其意义。方法采用免疫组织化学法和荧光实时定量PCR检测ESM-1在15份正常肝组织、15份癌旁组织及30份肝癌组织中的阳性表达率及mRNA水平，并分析肝癌组织ESM—1的表达情况与患者临床特征的关系；用酶联免疫吸附法测定相应肝癌患者及正常对照者血清中ESM—1的水平。计数资料采用x^2检验，计量资料采用两样本均数t检验及t’检验。结果在正常肝组织、癌旁组织及肝癌组织中，ESM-1的阳性表达率分别为0、40％（6／15）、80％（24／30）；mRNA水平分别为0．064±0．018、0．383±0．103、0．528±0．148，各组织中ESM1表达水平的差异有统计学意义（P〈0．05）。ESMI的表达与肝癌的TNM分期、血管侵犯显著相关（P〈0．01）。肝癌患者血清ESM-1的水平[（12．643±2．280）ng／ml]明显高于正常对照组[（4．660±1．172）ng／ml]，t=10．16，P〈0．05。结论肝癌患者ESM-1表达水平的增高可能在肝癌的发生、发展和转移中起重要作用。Objective To study the expression and significance of endothelial cell specific molecule- 1 （ESM-1） in hepatocellular carcinoma （HCC） tissue and serum from HCC patients. Methods The ESM-1 expression was evaluated by immunohistochemistry staining and quantitative real-time PCR in 30 tumor tissues from HCC patients, 15 surrounding non-cancerous hepatic parenchyma, and 15 normal controls. Serum levels of ESM-1 were also measured in the HCC patients and healthy controls by Enzyme-linked immunosorbent assay （ELISA）. Results ESM-1 was expressed in endothelium of 24 HCC tissues and 6 pericarcinomatous tissues, however, it was not expressed in normal liver tissues. The mRNA level of ESM-lwas 0.064 ± 0.018, 0.383 ± 0.103, 0.528 ± 0.148 in the corresponding tissues, respectively. The mRNA level of ESM-I in tissues was correlated with the TNM phase of HCC patients, tumor vascular invasion as well as metastasis. The serum ESM-I was （12.643 ± 2.280） ng/ml and （4.660 ± 1.172） ng/ml in hepatic cell carcinoma and normal controls, respectively. Conclusion The expression of ESM-1 is significantly upregulated in HCC, suggesting that ESM-1 may play an important role in the pathoigenesis of HCC.

关 键 词：癌 肝细胞 聚合酶链反应 免疫组织化学 内皮细胞特异性分子1 

分 类 号：R735.7[医药卫生—肿瘤]