淋巴管内皮透明质酸受体1在结直肠癌组织中的表达及其临床意义  

Expression of lymphatic vessel endothelial hyaluronan receptor-1 in human colorectal cancer and its clinical significance

作　　者：李占霞[1] 张国锋[1] 胡志前[2] 范跃祖[1] 

机构地区：[1]同济大学附属同济医院普通外科,上海200065 [2]第二军医大学长征医院普通外科

出　　处：《中华胃肠外科杂志》2009年第5期511-514,共4页Chinese Journal of Gastrointestinal Surgery

摘　　要：目的 明确淋巴管特异性标记物淋巴管内皮透明质酸受体1（LYVE-1）的表达情况，并探讨其表达与结直肠癌淋巴结转移及预后的关系。方法选取40例结直肠癌标本及其癌旁组织．采用实时定量PCR检测LYVE-1mRNA水平．采用免疫组织化学染色检测LYVE-1蛋白表达水平、淋巴管密度（LVD）及MVD．分析LYVE-1表达和LVD计数与淋巴结转移及患者预后的关系。结果结直肠癌组织中LYVE-1的表达和LVD计数明显高于正常组织（P〈0．05）。有淋巴结转移者LYVE-1表达水平和LVD计数均明显高于无淋巴结转移者（P〈0．05）。低LVD组术后复发率和3年生存率分别为46．7％和90．1％，高LVD组则分别为60．0％和56．7％（均P〈0．05）；而LYVE-1的表达则与术后复发及预后无明显相关（均P〉0．05）。结论LYVE-1特异性表达于淋巴管，与结直肠癌淋巴结转移密切相关：LVD可用于评价预后。Objective To examine the expression of lymph vessel endothelial hyaluronan receptor-1 （LYVE-1）in human colorectal carcinoma and to evaluate the relationship of LYVE-1 with lymph mode metastasis and prognosis. Methods Colonic cancer samples of 40 cases were collected. The expression of LYVE-1 was determined by RT-PCR and quantified by real-time quantitative PCR. LVD and MVD were detected by immunohistochemistry staining. The relationship of LYVE-1 and LVD with lymph mode metastasis and prognosis were analyzed. All the patients were followed up for at least 3 years. Results The expression of LYVE-1 and the count of LVD were significantly higher in tumor tissue than those in common colon tissue（P〈0.05）. In the majority of tumors, the higher count of LVD indicated lymphangiogenesis. The recurrence rates in low LVD group and high LVD group were 46.7% and 60.0% respectively（P〈0.05）. The survival rates in the above two groups were 90.1% and 56.7% respectively（P〈0.05）. No significant correlation was found between LYVE-1 and recurrence rate（P〉 0.05） or overall survival（P〉0.05）. Conclusion LYVE-1 indicates an increase of lymphangiogenesis in colorectal carcinoma and LVD can be used to evaluate the prognosis for colorectal cancer patients.

关 键 词：结直肠肿瘤 淋巴管内皮透明质酸受体1 淋巴管密度 

分 类 号：R735[医药卫生—肿瘤]