人参、三七提取物对Ras相关信号蛋白的影响  

Effect of Ginseng-Sanqi Extract on the Ras associated Signal Proteins

作　　者：田伟[1,2] 雷燕[1] 朱凌群[3] 陈可冀[1] 

机构地区：[1]中国中医科学院西苑医院,北京100091 [2]中国康复研究中心,北京100086 [3]北京中医药大学东直门医院

出　　处：《中国中西医结合杂志》2009年第9期802-805,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金项目(No.30672750)

摘　　要：目的探讨益气活血中药人参、三七提取物对血管内皮细胞血管生成信号蛋白血管内皮生长因子受体-2(VEGFR-2)、Ras及有丝分裂原活化蛋白激酶(MAPK)表达的影响。方法采用Western Blot检测人脐静脉内皮细胞(HUVEC)上VEGFR-2、Ras、MAPK蛋白表达和人参、三七提取物大、中、小剂量及碱性成纤维生长因子(bFGF)对其表达的影响。结果与空白对照组比较,中药大剂量组VEGFR-2及Ras蛋白表达明显增强,中药小剂量组MAPK及Ras蛋白表达明显上调,而bFGF组VEGFR-2、Ras及MAPK的表达均显著增强,差异均有统计学意义(P<0.05,P<0.01)。结论人参、三七提取物可促进血管生成信号通路上VEGFR-2、Ras及MAPK蛋白的表达,这可能是益气活血中药促进内皮增殖、产生促血管生成作用的基础。Objective To explore the effect of ginseng-sanqi extract（GSE） on the vascular endothelium growth factor receptor-2（VEGFR-2）,Ras and mitogen-activated protein kinase（MAPK） in VEGFR-2-Ras-MAPK signal pathway of angiogenesis in cultured human umbilical vascular endothelial cells（HUVECs）.Methods The protein expressions of VEGFR-2,Ras and MAPK in HUVEC and the effects of GSE（at different doses） and basic fibrin growth factor（bFGF） on them were detected by Western-Blot test.Results GSE can enhance the expression of angiogenesis signaling proteins（VEGFR-2,Ras,MAPK） to different extents,especially in the groups treated by bFGF or higher dose GSE,the levels showed significant differences to those in the blank group（P〈0.05）.Conclusion GSE can promote the expressions of VEGFR-2,Ras and MAPK in the angiogenesis signaling pathway,which may be the foundation of Chinese medicine for tonifying qi and activating blood circulation in promoting endothelial proliferation and angiogenesis.

关 键 词：人参 三七 信号转导 血管内皮生长因子受体-2 RAS 有丝分裂原活化蛋白激酶 

分 类 号：R285[医药卫生—中药学]