接种丛枝菌根对不同施氮水平下黄芪生理特性和营养成分的影响  

Effects of inoculating AM fungi on physiological characters and nutritional components of Astragalus membranaceus under different N application levels

作　　者：贺学礼[1] 刘媞[1] 赵丽莉[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《应用生态学报》2009年第9期2118-2122,共5页Chinese Journal of Applied Ecology

基　　金：河北省教育厅重点科研项目(ZH2006007);河北大学河北省自然科学基金预研项目(2006Y10)资助

摘　　要：以非灭菌土为生长基质,采用盆栽试验,研究了不同施氮水平下接种丛枝菌根(AM)真菌对黄芪生理特性和营养成分的影响.结果表明:AM真菌对不同施氮水平下黄芪的生长效果有显著影响,接种AM真菌能够提高黄芪根系菌根侵染率,但高施氮量抑制了AM真菌对黄芪根系的侵染.不同施氮水平下接种AM真菌可使黄芪生长量、叶片可溶性蛋白、可溶性糖含量及SOD、POD和CAT活性有不同程度的提高,且50mgN·kg-1土和100mgN·kg-1土施氮水平下接种株POD同工酶出现了新谱带,接种AM真菌对黄芪植株的黄酮、N和P含量也有一定促进作用.施氮量为50～100mgN·kg-1土时接种效果最佳.A pot culture with unsterilized soil as growth substrate showed that AM fungi had significant effects on the growth of Astragalus membranaceus （Fabaceae） under different N application levels. Inoculation with AM fungi promoted the AM infection of A. membranaceus roots, but high N application level suppressed the infection. Inoculating AM fungi increased the growth rate, soluble protein and sugar contents, and SOD, POD and CAT activities of A. membranaceus. Under 50 and 100 mg ·kg^-1 of N application, new bands of POD isozyme occurred in inoculated plants, and the contents of flavonoid, N, and P in A. membranaceus also had definite increase. The best inoculation effect was observed under the N application level of 50-100 mg N·kg^-1 soil.

关 键 词：AM真菌 施氮量 营养成分 生理特性 黄芪 

分 类 号：S567.239[农业科学—中草药栽培]