两种藻胆蛋白对人肝癌SMMC-7721细胞的光动力体外杀伤效应  

Photodynamic effect of two kinds of phycobiliproteins on human liver cancer cell line SMMC-7721 in vitro

作　　者：王源[1] 蔡春尔[1] 李柏林[1] 刘承初[1] 何培民[1] 

机构地区：[1]上海海洋大学,上海201306

出　　处：《生物工程学报》2009年第9期1417-1423,共7页Chinese Journal of Biotechnology

基　　金：国家高技术研究发展计划(863计划)(Nos.2006AA10Z323;2007AA09Z406);教育部水产种质资源发掘与利用省部共建重点开放实验室(No.KFT2008-12);上海市教委优势(重点)学科项目(No.S30701)资助~~

摘　　要：用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法测定提取自条斑紫菜的R-藻红蛋白(R-PE)和C-藻蓝蛋白(C-PC)经激光介导对人肝癌SMMC-7721细胞的杀伤率,并检测细胞凋亡率。结果显示在120mg/L浓度下,R-PE在氩离子激光器100J/cm2辐照下对应的细胞存活率为27%,C-PC在He-Ne激光器35J/cm2辐照下对应的细胞存活率为47%;单用2种激光器辐照对应的细胞存活率分别为65%和70%;单用2种蛋白处理细胞在72h后出现对细胞生长的显著抑制,最大抑制率分别为31%(R-PE,120mg/L)和27%(C-PC,250mg/L)。120mg/L的2种藻胆蛋白在对应激光辐照下,于照后8h达到细胞凋亡率最大值,分别为31.54%(R-PE)和32.54%(C-PC)。本实验证明条斑紫菜R-PE和C-PC具有可开发成为光敏剂的应用前景。We studied the effect of photodynamic therapy with phycobiliproteins on human liver cancer cells in vitro. With 3-（4, 5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay （MTT assay）, we used two phycobiliproteins, R-phycoerythrin （R-PE） and C-phycocyanin （C-PC） prepared from Porphyra yezoensis, to determine the killing rates and apoptosis rates of human liver cancer cells （SMMC-7721） mediated by laser. When the concentration of R-PE was 120 mg/L, the survival rate of human liver cancer cells was 27% after treated by Argon laser with 100 J/cm2 doses, while the survival rate in the control group （without adding R-PE） was 65%. When the C-PC concentration was 120 mg/L, the survival cell rate was 47% after treated by He-Ne laser with 35 J/cm2 dose, while the survival rate in the control group （without adding C-PC） was 70%. After handled only with these two kinds of phycobiliproteins for 72 h, the growth of cancer cells presented significant inhibition. The maximal inhibition rates reached up to 31% with R-PE （120 mg/L concentration） and 27% with C-PC （250 mgFL concentration） respectively. After irradiated by laser for 8 h the maximal cell apoptosis rates were 31.54% with R-PE and 32.54% with C-PC, respectively. It indicated that R-PE and C-PC extracted from Porphyra yezoensis could develop to new photosensitizers for cancer photodynamic therapy.

关 键 词：条斑紫菜 R-藻红蛋白 C-藻蓝蛋白 光敏作用 细胞凋亡 肝癌细胞 

分 类 号：R735.7[医药卫生—肿瘤]