8-硝基白杨素抑制HL-60细胞系增殖及促凋亡  

Effect of 8-nitrochyrsin on the proliferation and apoptosis of HL-60 cells

作　　者：岳军[1] 伍石华[2] 

机构地区：[1]邵阳医学高等专科学校生物化学教研室,湖南邵阳422000 [2]邵阳医学高等专科学校病理教研室,湖南邵阳422000

出　　处：《基础医学与临床》2009年第9期929-932,共4页Basic and Clinical Medicine

基　　金：湖南省教育厅优秀青年项目(06B087)

摘　　要：目的观察8-硝基白杨素(NOChR)抑制人急性髓性白血病细胞系HL-60细胞增殖和诱导凋亡作用。方法用MTT法检测HL-60细胞增殖活性;台盼蓝染色细胞计数法测定细胞存活率,绘制细胞生长曲线;DNA凝胶电泳观察HL-60细胞凋亡;流式细胞术检测HL-60细胞凋亡率。结果NOChR对HL-60细胞增殖具有抑制作用,呈浓度依赖性;NOChR显著抑制HL-60细胞生长;NOChR能有效地诱导HL-60细胞凋亡,呈浓度依赖性。结论NOChR具有抑制HL-60细胞增殖和诱导细胞凋亡作用。Objective To investigate the effect of 8-Nitrochyrsin on cell proliferation and cell apoptosis of HL-60 cells in vitro. Methods 8-Nitrochyrsin on proliferation of HL-60 cells was detected by MTT assay; The trypan blue exclusion method was used to count the number of viable and dead cells, then the survival rate and growth curve were achieved; DNA ladder bands were observed by DNA agarose gel electrophoresis; HL-60 cell apoptotic percentage was detected by flow cytometry with PI staining. Results 8-Nitrochrysin inhibited proliferation of HL-60 cells in a concentration-dependent manner; 8-Nitrochyrsin possess the inhibitory effect on the proliferation and growth of HL-60 cells; 8-Nitrochrysin possess the significantly effect of inducing apoptosis of HL-60 cells. Conclusion 8-Nitrochyrsin possess the inhibitory effect of the proliferation and growth of HL-60 cells and significantly induces apoptosis of HL-60 cells.

关 键 词：人急性髓性白血病 白杨素 8-硝基白杨素 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤]