春小麦杂种后代农艺性状和高分子量谷蛋白亚基组成分析  

AGRONOMIC TRAITS AND HMW-GS COMPOSITIOIN OF HYBRID OFFSPRING IN SPRING WHEAT

作　　者：王昆鹏[1] 刘爽[1] 郭世华[1] 

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019

出　　处：《内蒙古农业大学学报（自然科学版）》2009年第2期94-99,共6页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金(30460067);内蒙古自治区自然科学基金(200408020312)

摘　　要：为了客观评价春小麦杂种后代农艺和品质性状,采用DPS软件和SDS-PAGE技术分别对8个组合亲本及其杂种F4:5代8个农艺性状和高分子量谷蛋白亚基组成进行分析,结果表明,第1、3组合F4:5代平均有效分蘖数、结实小穗数、单穗籽粒数和单穗粒重均大于其双亲,第1、2、7组合F4:5代平均主茎穗长,第7组合结实小穗数,第2组合的单穗籽粒数和单穗粒重均大于其双亲。F4:5主穗单粒间HMW-GS组成基本相同;供试F4:5单株1、7+8和5+10亚基频率最高,分别为62.1%、51.8%和75.9%。8种亚基组合类型中,(1,7+8,5+10)的频率最高为20.7%。供试8个组合后代中,同一组合F4:5代单株Glu-1D位点亚基相同,Glu-1A、Glu-1B亚基类型不完全相同,同一株行的单株间亚基差异较小。此外,新克旱9号×陕253杂种F4:5单株出现与亲本不同的7+8亚基组。In order to evaluate the agronomic and quality traits of hybrid offspring in spring wheats, eight hybrid combined including their parents and F4：5 were used to analyse the agronomic traits and HMW - GS compositions using DPS software and SDS - PAGE techniques, respectively. The results indicated that effective tiller number, fruiting spikelets number, seed number of each ear and seeds weight of each ear of F4：5 were higher than that of their parents in the first and third compositions. Main ear long in 1,2,7 compositions, fruiting spikelets number in 7 combined, and seed number of each ear and seeds weight of each ear in the second combined were all higher than that of their parents. The HMW - GS compositions of single kernel from caulis spike were same. The frequencies of 1, 7 ＋8 and 5 ＋ 10 were highest than that of other subunits in tested F4：5 single plants, associated with 62.1% ,51.8% and 75.9%, respectively. Subunit combination ratio of 1, 7 ＋ 8, 5 ＋ 10 was the most than that of the others, with 20.7%. There was same subunit composition in the Glu - 1 D of F4,5 plant kernels from the same hybrid combination, but different in Glu - 1 A and Glu - 1 B of F4,5 plant kernels from the same hybrid combination. HMW - GS compositions from the same line was little differences. In addition, 7 ＋ 8 subunit type of HMW - GS presented in hybrid F4,5 kernels plants from Xinkehan9 × Shan253, which were different from their parents.

关 键 词：小麦 农艺性状 高分子量谷蛋白亚基 SDS-PAGE 

分 类 号：S512.132[农业科学—作物学]