小鼠睾丸高表达新基因Biot2-L表达谱分析及对睾丸发育影响的初步研究  

Analysis of Expression Pattern of a Novel Testis-highly Expressed Gene Biot2-L and the Primary Study on Its Role in Testis Development

作　　者：王昊[1] 张鹏[2] 王春婷[1] 

机构地区：[1]四川大学华西医院生物治疗国家重点实验室,成都610041 [2]四川省肿瘤医院放射治疗中心

出　　处：《四川大学学报（医学版）》2009年第5期853-856,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30801048)资助

摘　　要：目的分析小鼠可能的生精相关的新基因Biot2-L的组织表达谱,探讨其对睾丸发育的影响。方法利用生物信息学方法分析Biot2-L基因结构特征,不同物种间同源性情况。用RT-PCR、Real-time RT-PCR技术检测该基因在多种正常组织间的表达与定位,以及在睾丸发育过程中该基因表达的动态变化情况。结果获得基因全长编码序列,生物信息学分析显示编码Biot2-L蛋白中有一CCDC7结构域,在高等哺乳动物如人、大鼠、牛、猩猩等中发现同源蛋白,氨基酸序列同源性达50%以上,均含有CCDC7或相似结构域。RT-PCR证明Biot2-L基因在成年雄性C57小鼠的睾丸组织中大量表达,而在其他10种组织中不表达或低表达。Real-time RT-PCR检测显示Biot2-L基因在睾丸发育过程中呈有规律的动态变化,出生5周时Biot2-L基因开始表达,于7周时达到高峰,老年期时(50周)表达水平下调,表达规律与睾丸发育和生精过程相符。结论Biot2-L基因在成熟睾丸中高表达并随着睾丸发育过程,其表达水平呈有规律变化,提示该基因可能与睾丸发育及生精过程相关。bio-funetion on Objective To study on the expression pattern of a novel gene, Biot2-L, in mice and its primary the development of testis. Methods Bioinformatics analysis were applied to identify the structure characteristics and homologene of Biot2-L. RT-PCR and Real-time RT-PCR were used to analyze its expression profile in normal tissues and the time-dependent expression pattern during the testes sexual maturation. Results The complete sequence of Biot2-L was obtained. A conserved domain-CCDC7 was detected and there were homolog proteins in advanced mammals with about 50% identity by bioinformatics analysis. The highly expression of Biot2- L in adult testis but low or absent expressed in other tissues was observed. A regular change of Biot2-L expression level during the testis development and spermatogonia was demonstrated by the results of Real-time RT-PCR. The mRNA transcript appeared from the fifth week of postnatal development, and the expression level increased up to adulthood, but decreased when get older. Conclusion Biot2-L was highly expressed in adult testis and regular changed during the testis development, which suggested that the Biot2-L would be involved in the testis development and spermatogonia.

关 键 词：基因 睾丸 组织表达谱 

分 类 号：R363[医药卫生—病理学]