山东汉族关节病性银屑病与HLA—A、B等位基因相关性研究  

Relationship between human leukocyte antigen-A, -B alleles and psoriatic arthritis in Chinese Hart population from Shandong Province

作　　者：屈丽娜[1] 张福仁[1] 杨宝琦[1] 朱传福[2] 田洪青[1] 

机构地区：[1]山东省皮肤病性病防治研究所皮肤科,济南250022 [2]山东省血液中心

出　　处：《中华皮肤科杂志》2009年第9期604-606,共3页Chinese Journal of Dermatology

基　　金：山东省科技攻关项目（2007GG10002016）

摘　　要：目的探讨HLA—A、B等位基因与山东汉族关节病性银屑病及其临床特征的相关性。方法PCR-寡核苷酸探针杂交分型法（PCR—SSOP）对42例山东汉族关节病性银屑病患者（患者组）与90例健康献血者（对照组）进行HLA—A、B等位基因分型。结果两组比较，关节病性银屑病患者组B＊27等位基因频率（患者组为11％，对照组为1．8％，Pc〈0．01）、B＊57等位基因（分别为4．8％和0．4％，Pc〈0．05）及B＊13／B＊27杂合体（分别为8．5％和0．5％，Pc〈0．01）的出现频率明显高于对照组，A＊33等位基因频率（分别为0．6％和6．7％，Pc〈0．01）明显低于对照组。B＊27等位基因频率在关节病性银屑病脊柱受累组为80％，无脊柱受累组为22％（两组比较，P〈0．01）；外周附着点受累组为63％，无外周附着点受累组为23％（P〈0．05）。7例B＊13／27杂合体阳性者中5例（71％）有脊柱炎合并外周关节炎，而36例B＊13／27杂合体阴性者中有8例（22％），两组比较，r=6．73，P〈0．05。结论HLA—B＊27与山东汉族关节病性银屑病相关。Objective To investigate the relationship of human leukocyte antigen （HLA）-A, B alleles to psoriatic arthritis （PsA） and its clinical subtypes in Chinese Han population from Shandong Province. Methods Polymerase chain reaction-sequence specific oligonucleotide probe （PCR-SSOP） method was used to determine HLA-A, B alleles in 42 consecutive PsA patients and 90 healthy human controls of Han Nationality from Shandong Province. Results A significant increase was observed in the frequencies of HLA-B＊27 （11% vs 1.8%, P 〈 0.01 ）, B＊57（4.8% vs 0.4%, P 〈 0.01） alleles and B＊13/B＊27 heterozygote （8.5% vs 0.5%, P 〈 0.01 ） in PsA patients than those in the healthy controls, together with a decreased frequency of A＊33 allele （0.6% vs 6.7%, P 〈 0.001 ）. In PsA patients, there was a significant difference in the frequency of B＊27 allele between patients with spine involvement and those without （80% vs 22%, P 〈 0.01 ）, as well as between those with peripheral enthesis involvement and those without （63% vs 23%, P 〈 0.05）. Spondylitis incorporating peripheral arthritis was noted in 5 out of 7 patients positive for HLA B＊13/B＊27 heterozygote, compared to 8 out of 36 negative for HLA B＊13/B＊27 heterozygote （）X^2=6.73, P 〈 0.05 ）. Conclusion The HLA-B＊27 allele is correlated with PsA in Chinese Han population from Shandong Province.

关 键 词：关节炎 银屑病 等位基因 HLA—A抗原 HLA—B抗原 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]