TGFβ1与Smad 4基因在肝细胞癌中的表达及意义  

Expression and Significance of TGFβ1 and Smad 4 Gene in Human Hepatocellular Carcinoma Tissues

作　　者：程朋[1] 曾维政[2] 苏晓妹[1] 孙伟张[1] 谭艳[1] 谭勇[1] 张伶[1] 

机构地区：[1]成都军区总医院肿瘤科,610083 [2]成都军区总医院消化科,610083

出　　处：《实用癌症杂志》2009年第5期473-476,共4页The Practical Journal of Cancer

摘　　要：目的探讨TGFβ-Smad信号通路中TGFβ1与Co-Smad(Smad 4)与肝细胞癌的发生、发展的关系。方法采用免疫组化ABC法及原位杂交法(ISH),检测41例肝细胞癌组织与癌旁组织中TGFβ1、Smad 4蛋白、Smad 4 mRNA的表达,以5例外伤性肝破裂手术切除标本作为正常对照。比较HCC组与对照组及HCC癌旁中TGFβ1与Smad 4蛋白、Smad 4 mRNA表达的差异,并进行图像分析。结果正常对照组中TGFβ1呈阴性表达,Smad 4蛋白、Smad 4 mRNA均呈阳性表达;HCC组织中TGFβ1阳性表达率为78.0%(32/41),癌旁组织中为95.1%(39/41),两者比较差异显著(P<0.05);Smad 4蛋白在HCC组织中阳性表达率为48.8%(20/41),在癌旁组织中为78.0%(32/41),两者比较有显著性差异(P<0.01);Smad 4 m RNA在HCC组织中阳性表达率为51.2%(21/41),在癌旁组织中为73.1%(30/41),两者比较有显著性差异(P<0.05);与正常肝组织比较,HCC组织中Smad 4蛋白和Smad 4 mRNA阳性表达率均显著降低(P<0.05);Ⅰ、Ⅱ级HCC组织与Ⅲ、Ⅳ级HCC组织Smad 4 m RNA阳性表达存在显著差异(P<0.05),Ⅲ、Ⅳ级阳性表达率较Ⅰ、Ⅱ级降低;Smad 4蛋白的阳性面积百分比、光密度及Smad 4 m RNA的阳性面积百分比癌旁组织均显著高于HCC组织(P均<0.05)。HCC组织中Smad 4蛋白、Smad 4 m RNA表达与TGF-β1表达比较均存在显著差异(P<0.05),但均无显著相关性(P>0.05)。结论TGFβ1过表达、Smad 4表达缺失可能在肝细胞癌的发生、发展中发挥重要作用。Objective To investigate the correlation between the expression of TGFβ1, Smad 4 genes and the carcinogenesis and tumor progression of human hepatocellular carcinoma （HCC）. Methods IHC and ISH method was used to detect the expression of TGFβ1, Smad 4 protein and Smad 4 mRNA in the specimens from 41 HCC and the adjacent liver tissues. Tissues from 5 traumatic livers were used as normal control. Results TGFβ1 expression was negative, but Smad 4 protein and Smad 4 mRNA were positively expressed in normal liver tissues. The positive expression of TGFβ1, Smad 4 and Smad 4 mRNA was detected in the tissues from 32 cases（78.0%）,20 cases （48.8%） and 21 cases （51.2%） with HCC,and 39（95. 1%）,32 （78.0%） and 30 （73.1% ） adjacent liver tissues. The expression rate of TGFβ1, Smad 4 protein and Smad 4 protein was significantly different between the HCC and adjacent liver tissues （P 〈 0.05 ）. The expression of Smad 4 mRNA in HCC tissues was significantly lower in gradeⅢ - Ⅳthan that in grade Ⅰ- Ⅱ（ P 〈 0.05 ）. Conclusion The over - expression of TGFβ1 and the lower - expression of Smad 4 genes may play an important role in occurrence and progression of HCC.

关 键 词：肝细胞癌 TGFΒ1基因 SMAD 4基因 免疫组织化学 原位杂交 

分 类 号：R735.7[医药卫生—肿瘤]