牛膝引药作用对双氯芬酸钠抗佐剂型关节炎大鼠的影响  

Therapeutic Effect of Radix Achyranthis Bidentatae Guiding Function on Diclofenac Sodium Induced Adjuvant Rats Arthritis

作　　者：林月泉[1] 孙备[2] 杨士友[2] 吕凌[2] 刘羽[2] 

机构地区：[1]安徽中医学院,合肥230031 [2]安徽省药物研究所,合肥230022

出　　处：《中药新药与临床药理》2009年第5期408-411,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：安徽省自然科学基金项目(070413120)

摘　　要：目的研究牛膝对双氯芬酸钠治疗佐剂型关节炎大鼠的影响及其可能的作用机理。方法取Wistar大鼠45只,随机分为5组,每组9只,即正常对照组、模型对照组(给予等容量1%CM C-Na水溶液)、单用牛膝组(牛膝水提液含生药量5.0g.kg-1)、单用双氯芬酸钠组(双氯芬酸钠10mg.kg-1)及牛膝双氯芬酸钠复方组(牛膝水提液含生药量5.0g.kg-1+双氯芬酸钠10mg.kg-1)。采用弗式完全佐剂制备大鼠关节炎模型。运用容积法测定足跖体积,HPLC法测定双氯芬酸钠的药物浓度分布情况,ELISA法和放射免疫法测定体内PGE2和IL-1β的水平。结果牛膝双氯芬酸钠复方组的抗炎效果明显优于双氯芬酸钠组(P<0.05),药物组织分布比双氯芬酸钠组更为合理,体内PGE2及IL-1β水平也更接近正常水平(P<0.05)。结论牛膝能够提高双氯芬酸钠在炎症靶点的组织分布,并与其药效作用相吻合。其降低体内PGE2的作用可能和促进双氯芬酸钠向病灶组织分布蓄积有关。Objective To study the influence of guiding herb of Radix Achyranthis Bidentatae （RAB） on therapeutic effect of dielofenac sodium for rats with adjuvant arthritis （AA） and its possible mechansim, and to further explore the pharmacodynamic and pharmacokinetic changes after using diclofenac sodium together with RAB. Methods Forty - five Wistar rats were equally divided into 5 groups： normal group, model group, RAB （RAB water extract 5.0 g·kg^-1） group, diclofenac sodium （10 mg· kg^-1） group, and the combination group. The rat model was induced by Freud＇ s complete adjuvant. The foot edema was tested by volume method. HPLC was used to determine the diclofenac sodium concentration in foot tissue, and ELISA and radioimmunoas- say method were used to determine PGE2 and IL - 1β levels in vivo. Results In the combination group, the anti - inflammatory action was superior to that in diclofenac sodium group （ P 〈 0.05）, the tissue distribution of drugs was more reasonable than that in diclofenae sodium group （ P 〈 0.05）, and PGE2 and IL - 1 β levels in vivo were near to the normal. Conclusion RAB can increase the distribution of diclofenac sodium in target tissue, and the effect is correlated with its pharmacodynamic action. The reduction of PGE2 may have a certain relationship with the promotion of diclofenac sodium distribution in the target tissue.

关 键 词：牛膝 双氯芬酸钠 引药下行 佐剂型关节炎 组织分布 PGE2 IL-1Β 

分 类 号：R285.5[医药卫生—中药学]